Oxford Nanopore Technologies Limited

Isis Innovation Limited

Luke McNeill

Lakmal Nishantha Jayasinghe

Mark Bruce

Andrew John Heron

John Milton

Elizabeth Jayne Wallace

Ruth Moysey

Szabolcs Soeroes

Domenico Caprotti

James Anthony Clarke

Rebecca Victoria Bowen

David Alves

Stephen Robert Young

Pratik Raj Singh

Neil Roger Wood

Ramiz Iqbal Nathani

James White

Clive Gavin Brown

Christopher Joseph Schofield

Peter John Ratcliffe

Christopher William Pugh

Joseph Hargreaves Lloyd

Mihaela Misca

Antonino Puglisi

Jonathan Mark Gleadle

Anthony Clarke

David Antoni Alves

The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.

Mutant pore

The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Modified helicases

The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterizing target polynucleotides using helicases.

Method for controlling the movement of a polynucleotide through a transmembrane pore

The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Enzyme construct

The invention provides method of covalently coupling two or more moieties, the method comprising: (a) providing a first moiety having covalently attached thereto (i) at least one first linker comprising a first hybridizable region and (ii) at least one first group capable of forming a covalent bond; (b) providing a second moiety having covalently attached thereto (i) at least one second linker comprising a second hybridizable region capable of hybridizing to the first hybridizable region and (ii) at least a second group capable of forming a covalent bond with the first group; (c) contacting the first and second moieties under conditions that allow the first and second hybridizable regions to hybridize and link the moieties; and (d) exposing the linked moieties to conditions that allow the formation of a covalent bond between the first and second groups.

Hybridization linkers

A method of identifying an agent which modulates hydroxylation of hypoxia inducible factor (HIF), comprises contacting a HIF asparagine hydroxylase and a test substance in the presence of a substrate of the hydroxylase under conditions in which asparagine in the substrate is hydroxylated in the absence of the test substance; and determining hydroxylation of the substrate. Preferably the substrate is a HIF polypeptide comprising HIF-1&#x3b1;, a fragment thereof comprising Asn 803 of HIF-1&#x3b1; or a peptide analogue of HIF-1&#x3b1; or fragment thereof comprising an asparagine equivalent to Asn 803 of HIF-1&#x3b1; and wherein hydroxylation of Asn 803 or of a said equivalent asparagine is determined.

Assays for modulators of asparaginyl hydroxylase

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Oxford, United Kingdom

Luke McNeill