Novozymes A/s

The Procter Gamble Company

Luise Erlandsen

Jesper Vind

Allan Svendsen

Kim Borch

Carsten Hoerslev Hansen

Christel Thea Jorgensen

Carsten Peter Sonksen

Thomas Agersten Poulsen

Marco Malten

Lise Munch Mikkelsen

Carsten Horslev Hansen

Hanne Philbert Nielsen

Christian Lundager Gylstorff

Christian Jackel

Neil Joseph Lant

Hans Peter Heldt-Hansen

Ole Simonsen

Bitten Plesner

Carsten Hørslev Hansen

Anna Catharina Olsen Stenholm

Carsten Peter SÖNKSEN

Carsen Hoerslev Hansen

Christian JÄCKEL

Christel Thea Jørgensen

The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.

Detergent compositions

The present invention relates to lipase variants and methods of obtaining them. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Lipase variants and polynucleotides encoding same

The present invention relates to lipase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Enzymes tend to be inactivated during wash by a bleach catalyst in combination with a source of organic peroxyacids. The risk of enzyme inactivation by active bleach catalyst is reduced when the release of the enzyme into the wash solution is delayed. The enzyme stability during washing together with a bleach catalyst can be improved by applying a delayed-release coating to cores which comprise the enzyme.

Particulate composition

Methods and compositions for treating textiles and hard surfaces with compositions having specific lipases are described. The compositions have increased stability to oxidative degradation in particular due to bleach catalysts.

Compositions and methods for surface treatment with lipases

The inventors have developed a method using protein engineering to produce lipolytic enzymes having a relatively high activity for one ester bond in an amphiphilic substrate with two lipophilic groups) and a relatively low activity for the ester bond in an amphiphilic substrate with one lipophilic group, e.g. a relatively high phospholipase activity and a relatively low lysophospholipase activity.

Lipolytic enzymes

A method of screening lipolytic enzymes is used to identify candidates for a baking additive which can improve the properties of a baked product when added to the dough. The screening method involves contacting the enzyme with N-acyl phosphatidyl ethanolamine (APE) or N-acyl lyso-phosphatidyl ethanolamine (ALPE), and detecting hydrolysis of an ester bond in the APE or ALPE.

Method for selecting lipolytic enzyme

The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.

Lipolytic enzyme variants

Lipolytic enzymes variants

Variant lipolytic enzymes

Growing community of inventors

Frederikssund, Denmark

Luise Erlandsen