Isoplexis Corporation

Qiagen Sciences, LLC

Qiagen Waltham, Inc.

Luisa Andruzzi

Dona Hevroni

Mong Sano Marma

Angela DeLucia

Austin Ricker

Ben McNally

Timothy Pelletier

John Andrew Sheridan

Jimmy Adediran

Michel Georges Perbost

Elizabeth Carpenter

Minakshi Guha

Magnus Besev

Minakshi Gupta

The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3'-O position capped by a disulfide-based 3&#x2032;-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3&#x2032;-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

DNA sequencing reaction additive

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3'-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

Thiol-containing cleave reagents and oxidative wash

The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of photoprotective mixture of compounds as imaging reagents to improve stability and storage of fluorescent compounds, including but not limited to, nucleotides with fluorescent labels.

Photoprotective mixtures as imaging reagents in sequencing-by-synthesis

The present invention discloses methods of applications of indole-3-propionic acid, L-carnitine and O-acetyl-L-carnitine in one or more different reactive steps of a sequencing-by-synthesis workflow. The reactive steps employing these compounds include, but are not limited to, cleaving, imaging, incorporating bases and washing. The use of these new compounds provides improved sequencing performance including, but not limited to, lower error rates, higher sequence outputs and/or longer read lengths.

Scavenger compounds for improved sequencing-by-synthesis

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Concord, MA, United States of America

Luisa Andruzzi