Roche Vitamins Inc.

Hoffmann-la Roche Inc.

Dsm IP Assets B.v.

Novozymes A/s

Novozymes Als

Luis Pasamontes

Adolphus Van Loon

Markus Wyss

Andrea Tomschy

Kurt Vogel

Dirk Kostrewa

Hans-Peter Hohmann

Michel Tessier

Allan Svendsen

Mary-Helen Binger

Yuri Tsygankov

Martin Lehmann

S&oslash;ren Flensted Lassen

Soren Flensted Lassen

Martin Lehman

The present invention relates to phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG., are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such asphytase andphytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and/or improved thermostability.

Phytase variants

A process for the production of a modified phytase with a desired property improved over the property of the corresponding unmodified phytase is disclosed, as well as modified phytases, polynucleotides encoding modified phytases, and animal feed including modified phytases.

Modified phytases

Novel proteins of microorganism E-396 (FERM BP-4283) and the DNA sequences which encode these proteins have been discovered to provide an improved biosynthetic pathway from farnesyl pyrophosphate and isopentyl pyrophosphate to various carotenoids, especially zeaxanthin, astaxanthin, adonixanthin and canthaxanthin.

Fermentative carotenoid production

The present invention relates to phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG. are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such as Peniophora phytase and Aspergillus phytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and/or improved thermostability.

Novel proteins of Flavobacterium sp. R1534 and the DNA sequences which encode these proteins are disclosed which provide an improved biosynthetic pathway from farnesyl pyrophosphate and isopentyl pyrophosphate to various carotenoid precursors and carotenoids, especially &bgr;-carotene, lycopene, zeaxanthin and cantaxanthin.

Phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG. , are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such as Peniophora phytase and Aspergillus phytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and/or improved thermostability.

Phytase varinats

Novel proteins of Flavobacrerium sp. R1534 and the DNA sequences which encode these proteins are disclosed which provide an improved biosynthetic pathway from farnesyl pyrophosphate and isopentyl pyrophosphate to various carotenoid precursors and carotenoids, especially .beta.-carotene, lycopene, zeaxanthin and cantaxanthin. Processes are also provided for preparing zeaxanthin by culturing a transformed host cell containing an expression cassette that includes a polynucleotide having a DNA sequence which encodes the GGPP synthase of Flavobacterium sp. R1534 (crtE), the prephytoene synthase of Flavobacterium sp. R1534 (crtB), the phytoene desaturase of Flavobacterium sp. R1534 (crtI), the lycopene cyclase of Flavobacterium sp. R1534 (crtY), or the .beta.-carotene hydroxylase of Flavobacterium sp. R1534 (crtZ). The polynucleotide is substantially free of other polynucleotides of Flavobacterium sp. R1534. The process further includes isolating the zeaxanthin from such cells or the culture medium.

Novel proteins of Flavobacterium sp. R1534 and the DNA sequences which encode these proteins are disclosed which provide an improved biosynthetic pathway from farnesyl pyrophosphate and isopentyl pyrophosphate to various carotenoid precursors and carotenoids, especially .beta.-carotene, lycopene, zeaxanthin and cantaxanthin.

The invention provides an immunogenic polypeptide having the amino acid sequence

DNA encoding eimeria antigen

The invention provides an immunogenic polypeptide having the amino acid sequence ##STR1## and fragments thereof, which polypeptides are capable of inducing an immune response against Eimeria parasites, and the DNA encoding such polypeptides, as well as recombinant vectors and recombinant viruses containing the said DNA or fragments thereof and transformed microorganisms containing such vectors and viruses and coccidiosis vaccines comprising such polypeptides.

Growing community of inventors

Trimbach, Switzerland

Luis Pasamontes