Illumina Corporation

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Ludovic Vincent

Andrew Antony Brown

Wayne N George

Sang Ryul Park

Lewis J Kraft

Brian Douglas Mather

Boyan Boyanov

M Shane Bowen

Jeffrey S Fisher

Maria Candelaria Rogert Bacigalupo

Justin Fullerton

Sahngki Hong

Alexandre Richez

Sean M Ramirez

Wei Wei

Edwin Li

David Louis Heiner

John M Beierle

James M Tsay

Kevan Samiee

Peyton Shieh

Mathieu Lessard-Viger

Anmiv Prabhu

Scott William Bailey

Tyler Jamison Dill

Robert Yang

Naiqian Zhan

Yuxiang Huang

Kristina Munoz

Leonid Malevanchik

Samantha Schmitt

Sunmin Ahn

Valerie Uzzell

Arvin Emadi

Hai Quang Tran

Dajun Yuan

Xavier Von Hatten

Hong Xie

Gianluca Andrea Artioli

Julia Morrison

Tara Bozorg-Grayeli

Hayden Black

Maxwell Zimmerley

Krystal Sly

Innsu Daniel Kim

Sang Park

Sojeong Moon

Ryan Sanford

Vanessa Montaño Machado

Provided herein include various examples of an apparatus, a sensor system and examples of a method for manufacturing aspects of an apparatus, a sensor system. The method may include forming bumps on a surface of one or more electrical contacts, where the one or more electrical contacts are accessible on an upper surface of a die, where the die is oriented on a substrate, and where the electrical contacts comprise bonding pads. The method may also include coupling one or more additional electrical contacts to the one or more electrical contacts, where the coupling comprises wire-bonding each additional electrical contact of the additional electrical contacts to one of the one or more electrical contacts accessible on the upper surface of the die, via a portion of the bumps on the surface of the one or more electrical contacts, thereby forming wire-bonded connections.

Enabling sensor top side wirebonding

A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.

Flow cell including a heteropolymer

An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

Flow cells

A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

Flow cell package and method for making the same

An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

Protective surface coatings for flow cells

Growing community of inventors

San Diego, CA, United States of America

Ludovic Vincent