Centre National De La Recherche Scientifique

Sorbonne Université

Institut Curie

Ecole Normale Superieure De Paris

Université Pierre Et Marie Curie

Ecole Normale Superieure

Paris Sciences Et Lettres

Paris Sciences Et Lettres-quartier Latin

Universite Pierre Et Marie Curie—paris 6 (Upmc)

Ludovic Jullien

Thomas Le Saux

Arnaud Gautier

Jérôme Querard

Vincent Croquette

Franck Perez

Agathe Espagne

Chenge Li

Charlie Gosse

Ruikang Zhang

Laurence Signon Colonna Bozzi

Agnès Pellissier-tanon

Annie Lemarchand

Raja Chouket

Herve Lemarchand

Samantha Albright

Pencheng Wang

Nath Sarang

Croquette Vincent

Agnés Pelissier-Tanon

Lemarchand Annie

Le Saux Thomas

Isabelle Aujard

Kais Zrelli

An apparatus for performing photochemical measurements on a liquid or liquid-containing sample includes a light generating system, an optical system configured for directing light from the light generating system towards the sample and a sensing system for measuring at least one optical property of the sample, wherein the light generating system is configured for generating light with intensity levels adjustable within a range, spanning at least three and preferably at least five orders of magnitude; and in that the optical system is configured for directing light from the light generating system towards the sample so as to generate a substantially uniform light intensity level, within a cylindrical region of the sample having a height larger than or equal to five time its radius.

Apparatus for performing photochemical measurements on a liquid or liquid-containing sample

Fluorescent labeling of proteins. In particular, membrane-impermeant fluorogenic chromophores being capable of binding reversibly a functional derivative of a Photoactive Yellow Protein (PYP), or a functional fragment thereof, for fluorescently labeling biological molecules of interest, preferably proteins of interest. Especially, 4-hydroxybenzylidene-rhodanine (HBR) analogs of formula (II) as membrane-impermeant fluorogenic chromophores. 

Membrane-impermeant fluorogenic chromophores

A method is provided for detecting a fluorescent species that is reversibly photoswitchable at high frequency, and more precisely to a method for detecting at least one reversibly photoswitchable fluorescent species, including a step consisting in illuminating a sample containing a reversibly photoswitchable fluorescent species with a first illuminating light beam, of wavelength &#x3bb;, and periodically modulated at an angular frequency &#x3c9;, and with a second illuminating light beam of wavelength &#x3bb;different from &#x3bb;, which is periodically modulated at the angular frequency &#x3c9;, the second illuminating light beam being modulated in antiphase with respect to the first illuminating light beam.

Method for detecting fluorescent species that are reversibly photoswitchable at a high frequency

A method for detection of at least one reversibly photo-convertible fluorescent species, comprises the following steps: a) illumination of a sample comprising said or at least one of the reversibly photo-convertible fluorescent species by a periodically modulated illuminating light; and b) detection of fluorescent emission emitted by the sample thus illuminated; wherein the method further comprises the following step: c) extraction of the amplitude of the intensity component of the fluorescent emission exhibiting the same periodicity as the periodically modulated illuminating light and a phase quadrature with respect to the same; and wherein the mean intensity of the illuminating light and the modulation frequency of the same are chosen to maximize the amplitude of the intensity component of the fluorescent emission.

Method for detection of a reversibly photo-convertible fluorescent species

Disclosed is a functional derivative of a Photoactive Yellow Protein (PYP), or a functional fragment thereof, for fluorescently labelling particles, e.g. proteins, or surfaces, which is capable of binding reversibly a fluorogenic chromophore of formula (I), and which is capable of enhancing the brightness of the fluorogenic chromophore upon complexation thereto; and of inducing the spectral shift of the fluorogenic chromophore through the ionization of an auxochromic group thereof.

Fluorogen activating and shifting tag (FAST)

A method for separating a chemical or biological compound present in a mixture of similar compounds by diffusion in a medium, the method comprising reacting the compounds of the mixture with a component (P) present in the medium to obtain products (Q), wherein the reactions C+P&#x2192;Qare reversible and have kinetic constants kand k, and applying to the medium a varying field to which the compounds are sensitive, wherein the period of the field and the concentration of component (P) in the medium are determined from the kinetic constants (k,k) of the compound (C) to be separated in order to establish resonance condition between said reactions and the field and for giving compound C an apparent diffusion coefficient (D) in the medium that is maximum.

Method for separating a chemical or biological compound in a mixture of similar compounds by diffusion in a medium such as a gel

Growing community of inventors

Arcueil, France

Ludovic Jullien