The United States of America as Represented by the Secretary of

Other

Nippon Zeon Company, Ltd.

Lucy F Lee

Richard L Witter

Keyvan Nazerian

Noboru Yanagida

Henry D Hunt

Ping Wu

Aly M Fadly

Ryohei Ogawa

Shigeto Yoshida

Yi Li

Zhizhong Cui

A nucleotide sequence encoding the gp82 polypeptide of Marek's disease virus is disclosed. Also disclosed are recombinant viruses which are useful as vaccines for protecting against Marek's Disease, preferably containing two or more genes encoding Marek's Disease Virus antigens such as glycoprotein B and glycoprotein gp82, under the control of a poxvirus promoter within a region of the DNA of fowlpox virus which is not essential for virus growth. Also provided is a vaccine exhibiting a synergistic immunoprotective effect, comprising a recombinant fowlpox virus expressing Marek's Disease Virus gB protein in combination with turkey herpesvirus. A method of immunizing poultry, comprising administering any of the disclosed vaccines, is also provided.

Marek's disease virus vaccines for protection against Marek's disease

The envelope (env) gene from avian leukosis virus subgroup J (ALV-J) strain Hc1 has been isolated, sequenced and cloned into an expression vector. The ALV-J Hc1 env gene and expressed protein are useful for development of diagnostic assays to detect Hc1-specific nucleic acid and proteins and for eliciting an immune response in chickens.

Avian leukosis virus subgroup J envelope gene product for diagnosis and

A recombinant fowlpox virus is disclosed which is useful as a vaccine for protection against Marek's Disease. The recombinant virus preferably contains a gene for one or more Marek's Disease Virus antigens such as glycoprotein B homologue, glycoprotein C homologue, glycoprotein D homologue, glycoprotein H homologue and tegument proteins, under the control of a poxvirus promoter within a region of the DNA of fowlpox virus which is not essential for virus growth.

Recombinant fowlpox vaccine for protection against Marek's disease

The combination of monoclonal antibodies 11A25 and 11E32, which recognize two distinct antigenic sites on the 62-kd glycoprotein of reticuloendotheliosis virus (REV) in ELISA, enables an increase in the sensitivity of an ELISA assay for subtype 2 REV over what was heretofore possible. The combination of MAbs is useful for diagnosing REV antigen and antibody in poultry flocks and for detecting new strains and variants of the viral subtype.

Monoclonal antibody immunoassay kit for avian reticuloendotheliosis virus

Growing community of inventors

East Lansing, MI, United States of America

Lucy F Lee