Dekalb Genetics Corporation

Monsanto Technology LLC

Lucille B Laccetti

Emil M Orozco, Jr

David McElroy

Albert P Kausch

David A Walters

Paul S Chomet

Paul C Anderson

Michael T Mann

Peggy G Lemaux

Thomas R Adams

T Michael Spencer

James V O'Brien

William J Gordon-Kamm

Nancy M Houmard

Catherine J Mackey

Richard J Daines

Peter M Orr

Nancy G Willetts

Sheryl A Chambers

Thomas B Rice

William G Start

Michael A Stephens

Mary L Mangano

Emil M Orozco

Donald S Walters

Christopher E Flick

Susan J Zachwieja

Clayton S Vetsch

Kimberly Glassman

Albert Kausch

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs thebar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof

The current invention provides the promoter of thenuclear gene encoding chloroplast-localized fructose-1,6-bisphosphate (F16BP) aldolase. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the chloroplastic F16BP aldolase promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Maize chloroplast aldolase promoter compositions and methods for use thereof

The current invention provides the GRP (ZMGRP) promoter. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the ZMGRP promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Maize glycine rich protein promoter compositions and methods for use thereof

The current invention provides the maize RS81 promoter. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the RS81 promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Maize RS81 promoter and methods for use thereof

The current invention provides the maize RS324 promoter. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the RS324 promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Maize RS324 promoter and methods for use thereof

Growing community of inventors

Groton, CT, United States of America

Lucille B Laccetti