Universitè Laval

Organogenesis, Inc.

Universitéde Montreal

Lucie Germain

François Auger

François Bergeron

Francedillaois A Auger

Bertrand Lussier

Louis-Mathieu Stevens

Nazrul Islam

Sheila Laverty

Daniel Dupuis

Eve Langelier

Marc Bouchard

Jean Lamontagne

Anne-Marie Belzil

Francois A Auger

Julie Tremblay

Rejean Cloutier

Denis Rancourt

Francine Goulet

Pierrot Tremblay

Louis Lafleur

Nicolas L'Heureux

Mahmoud Rouabhia

Sylvain Guerin

Stephanie Bouchard

Constance Guillemette, Legal Representative

Charles Roberge

Cindy J Hayward

Murielle Remy-Zolghadri

Julie Fradette

François A Auger

The present invention relates a method for isolating four cell types from a single umbilical cord as pure cultures. These cell lines (epithelial cells, fibroblasts, smooth muscle cells and endothelial cells) can be characterized and utilized in experimental models and for therapeutic purposes. Particularly, the umbilical cells isolated herein are used to form a tissue replacement or engineered living composition. Also, the isolated umbilical cells of the invention may have the potential of progenitor cells.

Method of isolating cells from umbilical cord

A method and a device () for reorganizing the fibers of a matrix in a living tissue sheet (S) by inducing controlled mechanical constraints in the living tissue (S) sheet thus causing the fibers of the matrix to be aligned parallel to the strain orientation. The sheet (S) is held in a stretched state until the fibers set in place.

Method and apparatus for inducing controlled mechanical constraints in a tissue construct

A method for preparing a human or animal tissue by applying a compressive force to a stack of sheets of living tissue thereby inducing adjacent layers to fuse or adhere to each other. The force is applied in direction normal to the surface of the tissue. A multi-layer tissue produced by the method described above can also possess at least two different types of sheets and/or consist essentially of between two and twelve sheets of living tissue. The method can also be used to prepare a planar tissue that can further be incorporated in a multi-layer tissue construct. The methods and tissues described herein are useful for the preparation of engineered tissues.

Method for preparing engineered tissue

The present invention relates to connective tissue substitute implant and method of preparation thereof. The implant is essentially composed of two bone anchors joined at the proximal ends by matrix layers and/or filaments coated by supplementary biocompatible matrix coating layer which can contain living stem cells isolated from injured connective tissue.

Connective tissue substitutes, method of preparation and uses thereof

A contractile smooth muscle cell construct and a method for preparing the smooth muscle cell construct are described. The smooth muscle cell construct is comprised of (i) smooth muscle cells that have been cultured in vitro under conditions to allow the formation of a sheet of smooth muscle cells and (ii) an endogenous fibrous matrix formed by the smooth muscle cells, wherein the smooth muscle cell construct retains the ability to contract in response to vasoactive agonists. The smooth muscle cell construct may be prepared in planar or tubular form and can be used to study or evaluate the contractile responses of smooth muscle cells.

Production of a contractile smooth muscle

A process for culturing and generating a chimeric cell culture, in particular chimeric epithelium, is disclosed. The chimeric epithelium can be used to treat skin trauma such as burn victims. Autologous epithelial grafts have been used on burn patients although this requires that the patient's cells are cultured and expanded in vitro which generally takes four to five weeks. The chimeric epithelium of the present invention is composed of cells that are both autologous and allogeneic to the host. Therefore, the allogeneic cells can be maintained in a cell bank and co-cultured with autologous host cells when needed. This significantly reduces the time required (by up to 50%) for autologous cell expansion and culture prior to grafting. Furthermore, it has been demonstrated that the allogeneic cells are passively eliminated from the graft without rejection of the total graft.

Chimeric sheets of epithelial cells

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St-Augustin, Canada

Lucie Germain