Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences

Luchang Wan

Qi Wang

Qiang Yao

Jin Li

Zhiyuan Gong

Zhaohui Liu

Xiao Liu

Peng Yang

Jiandong Han

Pengfei Ren

Haixia Ren

Tao Sun

A method for producing laccase by liquid fermentation ofcomprises the following steps of: (1) inoculating strains ofinto the PD liquid fermentation medium to perform activation culture and obtain a seed liquid; (2) inoculating the seed solution into a liquid fermentation medium to perform liquid fermentation culture, then adding an expansin solution to perform further culture, separating the supernatant to obtain a ferment solution; (3) extracting laccase from the ferment solution. A plant expansin and an optimized fermentation method are applied for producing laccase by liquid fermentation of, which increases the liquid fermentation output of laccase ofremarkably, by more than 3 times in comparing with traditional fermentation production methods.

[object Object]

Growing community of inventors

Jinan, China

Luchang Wan