University of Washington

Massachusetts Institute of Technology

Lucas Nivon

David Baker

Alice Y Ting

Lauren Carter

Gustav Oberdorfer

Florian Richter

Tamuka Chidyausiku

Daniel Shao-Chen Liu

Enrique Marcos

Beta-sheet forming polypeptides at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to the amino acid sequence selected from the group consisting of SEQ ID NOS:1-24 are disclosed, together with their use and methods for designing beta-sheet forming polypeptides.

De novo designed non-local beta sheet proteins

The present disclosure provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs (e.g., lipoic acid analogs comprising a resorufin moiety) recognized by lipoic acid ligase and lipoic acid ligase mutants. Also provided herein is a method of imaging protein-protein interaction via a reaction mediated by lipoic acid ligase.

Methods and compositions for protein labeling using lipoic acid ligases

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Seattle, WA, United States of America

Lucas Nivon