Quest Diagnostics Investments, Inc.

Luca Jacky

Michelle M Tabb

Maurice Exner

Michael Aye

Huong Mai

Yin-Peng Chen

Jules Chen

Emberlee Eleazar

Provided herein are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids.

Direct amplification and detection of viral and bacterial pathogens

Provided are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Further provided are reagent mixtures that allow for direct amplification of a sample, without the step of nucleic acid extraction.

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Orange, CA, United States of America

Luca Jacky