University of California

The United States of America, As Represented by the Secretary, Department of Health and Human Services

Luc Jaeger

Bruce Allen Shapiro

Kirill Andreevich Afonin

Eckart Bindewald

Mathias D Viard

Yaroslava Georgievna Yingling

Wojciech Kasprzak

Isil Severcan

Cody Geary

Maria L Kireeva

Mikhail Kashlev

Wade W Grabow

Arti N Santhanam

Paul J Zakrevsky

Disclosed are nanostructures comprising a ribonucleic acid (RNA) scaffold comprising a hexameric tetrahedral core. The tetrahedral core may comprise four hexameric RNA nanorings linked together. Related pharmaceutical compositions, methods of modulating the expression of a target gene in a mammal, methods of treating or preventing a disease in a mammal, and methods of producing a hexameric tetrahedral RNA nanostructure are also disclosed.

Hexameric tetrahedral RNA nanostructures

The instant invention provides polyvalent RNA nanoparticles comprising RNA motifs as building blocks that can form RNA nanotubes. The polyvalent RNA nanoparticles are suitable for therapeutic or diagnostic use in a number of diseases or disorders.

RNA nanoparticles and nanotubes

A method is provided for generating RNA nanoparticles having modified nucleotides and/or having increased nuclease resistance where the RNA nanoparticles are formed cotranscriptionally by T7 RNA polymerase in the presence of manganese ions.

Co-transcriptional assembly of modified RNA nanoparticles

Auto-recognizing therapeutic R/DNA chimeric nanoparticles (R/DNA NP) are described that are pairs of DNA/RNA hybrids where the DNA molecules have complementary toehold sequences that promote the re-association of the R/DNA NPs when mixed resulting in the formation of RNA/RNA duplexes that act as siRNAs.

Auto-recognizing therapeutic RNA/DNA chimeric nanoparticles (NP)

Growing community of inventors

Goleta, CA, United States of America

Luc Jaeger