Danisco US Inc.

The Procter Gamble Company

Louise Wallace

David Aaron Estell

Sang-Kyu Lee

Andrew Shaw

Eugene Steven Sadlowski

Hiroshi Oh

Scott Douglas Power

Sandra W Ramer

William Albert Cuevas

Ronaldus W J Hommes

Amr R Toppozada

Ronaldus Wilhelmus Joannes Hommes

Luis Gustavo Cascao-Pereira

Richard Ray Bott

Frits Goedegebuur

Benjamin S Bower

Wolfgang Aehle

Michael Jay Pepsin

Bradley R Kelemen

Thijs Kaper

Slavko Kralj

Igor Nikolaev

Gudrun Vogtentanz

Sander Van Stigt Thans

Mats Sandgren

Carol A Requadt

Regina Chin

Jonathan Caspi

Johannes Franciscus Thomas Van Lieshout

David E Estell

Johan Van Lieshout

Ronald Hommes

William A Cuevas

The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained fromsp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from. In still further preferred embodiments, the neutral metalloprotease is a variant of theneutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the theneutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

Use and production of storage-stable neutral metalloprotease

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.

Cellulase variants with improved expression, activity and stability, and use thereof

Alpha-amylases from(AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.

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An &#x3b1;-amylase from(AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.

The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained fromsp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from. In still further preferred embodiments, the neutral metalloprotease is a variant of theneutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of theneutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

Thermostable neutral metalloproteases

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Menlo Park, CA, United States of America

Louise Wallace