Arizona State University

Louis Schoettle

Joseph Blattman

Hao Yan

Xixi Wei

Methods for incorporation of unique bowtie-barcodes into a nucleic acid origami nanostructure (FIG.). In particular, provided herein are methods that facilitate pairing and analysis of nucleic acids from individual cells using, for example, high-throughput next-generation sequencing.

High throughput oil-emulsion synthesis of bowtie barcodes for paired mRNA capture and sequencing from individual cells

Methods for construction of DNA origami nanostructures, as well as for binding, isolation, linking, and deep sequencing information, such as both of TCR alpha and beta CDR3 mRNA, from individual cells within a mixed population of cells without the need for single cell sorting (FIG.).

Methods for obtaining information from single cells within populations using DNA origami nanostructures without the need for single cell sorting

Methods for construction of DNA origami nanostructures, as well as for binding, isolation, linking, and deep sequencing information, such as both of TCR alpha and beta CDR3 mRNA, from individual cells within a mixed population of cells without the need for single cell sorting.

Growing community of inventors

Tempe, AZ, United States of America

Louis Schoettle