Hoffmann-La Roche Inc.

The Perkin-Elmer Corporation

Louis M Mezei

Kary B Mullis

Richard A Leath

Joseph T Widunas

Larry Johnson

Timothy J Wennberg

There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.

System for automated performance of the polymerase chain reaction

Apparatus and method for performing automated amplification of nucleic

Growing community of inventors

Madison, WI, United States of America

Louis M Mezei