Codexis, Inc.

Shell Oil Comapny

Precigen, Inc.

Other

Shell Internationale Research Maatschappij, B.v.

Louis Clark

Dipnath Baidyaroy

Fernando Valle

Lisa Marie Newman

Sally Rhiannon Postlethwaite

Xiyun Zhang

Jie Yang

Behnaz Behrouzian

Catherine M Cho

Kristian Karlshoej

Douglas A Hattendorf

Jonathan Vroom

Lorand Szabo

Robert G McDaniel

Attila Andor

Jennifer Shock

Kaman Chan

Yoram Barak

Rama Voladri

Xinhua Zhao

Shiwei Song

Onorato Campopiano

Michael D Clay

David Elgart

Erik Jan De Vries

Kripa Rao

James Kealey

Lily Yuin Chao

Kevin Lee Dietzel

Mark Anton Held

Na Trinh

Grayson Wawrzyn

Jeffrey Orth

Matthias Helmut Schmalisch

Erika Noel Segraves

Yihui Zhu

Svetlana Balatskaya

Xuezhi Li

Trish Choudhary

Janos Torok

Scott McVicar

Kheng Lin Tan

Christopher Cheyney Farwell

Jovana Nazor

Robert Osborne

Charlene Ching

Claus M Krebber

Smita Shankar

Kenneth W Mitchell

Patricia Choudhary

Robert Mcdaniel

Jennifer L Shock

Genetically modified microorganisms that have the ability to convert carbon substrates into chemical products such as isobutanol are disclosed. For example, genetically modified methanotrophs that are capable of generating isobutanol at high titers from a methane source are disclosed. Methods of making these genetically modified microorganisms and methods of using them are also disclosed.

Methods and microorganisms for the fermentation of methane to multi-carbon compounds

Genetically modified microorganisms that have the ability to convert carbon substrates into multicarbon products. Methods of making these genetically modified microorganisms and methods of using them. Vectors encoding enzymes for use in converting carbon substrates into multicarbon products.

This invention provides microbial organisms, particularly yeasts such as, that have one or more disrupted genes. The gene disruption(s) may yield improved production of fatty acyl-CoA derivatives.

Gene disruptants producing fatty acyl-CoA derivatives

The invention generally relates to the production of a fatty alcohol composition from recombinant microbial cells. The fatty alcohols are produced by expressing a gene encoding a heterologous fatty alcohol forming acyl-CoA reductase ('FAR'); a gene encoding a heterologous thioesterase ('TE') gene and a gene encoding an acyl-CoA synthetase (&#x201c;ACS&#x201d;).

Production of fatty alcohols from engineered microorganisms

Recombinant microorganisms are provided which have been engineered to produce fatty alcohols. Also provided are recombinant microorganisms which comprise a heterologous polynucleotide encoding a fatty alcohol reductase enzyme and an introduced polynucleotide encoding a &#x3b2;-ketoacyl acyl carrier protein synthase.

Region shuffling methods for efficiently introducing diversity and exploring sequence space are described. Libraries produced directly from these methods contain high fractions of protein variants harboring multiple beneficial mutations. Typically, the methods produce these variants efficiently without the need for sequencing beneficial mutants identified at intermediate stages of the process.

Protein variant generation by region shuffling

Genetically engineered cells and microorganisms are provided that produce fatty alcohols and fatty acids. In particular, engineered microbial cells comprise a modified native gene having &#x3b2;-ketoacyl-acp synthase activity.

Modified native beta-ketoacyl-ACP synthases and engineered microorganisms

The invention provides recombinant GH61 proteins obtained from, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated fromsupernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, &#x3b2;-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.

Use of glycohydrolase 61 protein variants with improved thermostability for processing cellulose

Recombinant bacterial microorganisms are provided which comprise heterologous fatty acyl reductases ('FAR') polypeptides wherein said microorganisms have been engineered to produce increased amounts of saturated fatty alcohols and methods of making saturated fatty alcohols using the recombinant bacterial microorganisms.

Production of saturated fatty alcohols from engineered microorganisms

The present invention provides promoters derived from a filamentous fungus. These promoters have application in the fields of molecular biology, microbiology, fungal genetics and production of biofuels and other products.

Expression constructs comprising fungal promoters

The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.

Cellobiohydrolase variants

The invention relates to expression of a recombinant C1 &#x3b2;-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting cellobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 &#x3b2;-glucosidase, operably linked to heterologous promoter, under conditions in which &#x3b2;-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said &#x3b2;-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

Recombinant C1 beta-glucosidase for production of sugars from cellulosic biomass

The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same.

Fatty alcohol forming acyl reductases (FARs) and methods of use thereof

The invention relates to recombinantly produced &#x3b2;-glucosidase variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant &#x3b2;-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target &#x3b2;-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.

Beta-glucosidase variants

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San Francisco, CA, United States of America

Louis Clark