Hoechst Schering Agrevo Gmbh

Planttec Biotechnologie Gmbh

Institut Fur Genbiologische Forschung Berlin Gmbh

Other

Schering Aktiengesellschaft

Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Institut Fur Genbiologische

Lothar Willmitzer

Uwe Sonnewald

Jens Kossmann

Wolf-Bernd Frommer

Michael Emmermann

Bernd Muller

Joachim Kopka

Klaus Geider

Gebhard Geier

Antje Von Schaeven

Marina Stratmann

Mario Rocha-Sosa

Jurgen Logemann

Evert Jacobsen

Richard G F Visser

Horst L&ouml;rz

Stephanie L&uuml;tticke

Manuela Rober

Bernd Muller-Rober

Monika Chodasiewicz

Manuela R&ouml;ber

Sylwia Kierszniowska

Jens Kossman

Bernd M&uuml;ller-R&ouml;ber

Alexander Lüdemann

Rainer Hofgen

Josef Schell

Aleksandra Skirycz

Daniel Veyel

Christoph Kr&ouml;ger

Ute Roessner-Tunali

Alisdair Fernie

Ewa Urbanczyk-Wochniak

Joachim Selbig

This invention relates to a method of determining ligands of macromolecules, said method comprising or consisting of (a) subjecting a sample comprising (i) complexes formed by said macromolecules and said ligands and (ii) unbound ligands to a method which separates said complexes from said unbound ligands; (b) releasing ligands from complexes obtained in step (a); and (c) subjecting the released ligands obtained in step (b) to a chemical analysis method, thereby determining said ligands of said macromolecules.

Ligand identification by co-fractionation

Nucleic acid molecules are described, which encode debranching enzymes from , as well as transgenic plant cells and plants in which an amylopectin with modified properties is synthesized due to the expression of a debranching enzyme from or due to the inhibition of such an endogeneous debranching enzyme activity.

Nucleic acid molecules coding for debranching enzymes from maize

Nucleotide sequences encoding enzymes that alter the carbohydrate concentration and composition in plants are described. The sequences encode branching enzymes which alter the amylose/amylopectin ratio in the starch of plants.

Nucleotide sequences encoding enzymes that alter the carbohydrate concentration and composition in plants

DNA which leads to the formation of polyfructans (levans), plasmids containing this DNA, as well as processes using plasmids for preparing transgenic plants with polyfructan (levan) expression.

DNA sequences which lead to the formation of polyfructans (levans), plasmids containing these sequences as well as a process for preparing transgenic plants

Nucleic acid molecules are described, which encode debranching enzymes from maize, as well as transgenic plant cells and plants in which an amylopectin with modified properties is synthesized due to the expression of a debranching enzyme from maize or due to the inhibition of such an endogeneous debranching enzyme activity.

Plasmids are described having DNA sequences that after insertion into the genome of the plants cause changes in the carbohydrate concentration and the carbohydrate composition in regenerated plants. These changes can be obtained from a sequence of a branching enzyme that is located on these plasmids. This branching enzyme alters the amylose/amylopectin ratio in starch of the plants, especially in commercially used plants.

Plasmids containing DNA-sequences that cause changes in the carbohydrate concentration and carbohydrate composition in plants, as well as plant cells and plants containing these plasmids

Plasmids containing DNA sequences which when inserted into the genome of a plant modify the carbohydrate or protein concentration and the carbohydrate or protein composition, and plant cells and plants that contain these plasmids. DNA sequences located in plasmids reduce ADP-glucose-pyrophosphorylase activity in the plant and thereby the starch concentration, while at the same time increase the mono- and oligosaccharide concentration. Other DNA sequences in plasmids reduce or increase the protein concentration. Plants that contain these plasmids are suitable inter alia for the extraction of sugar or as protein-enriched food or fodder.

Plasmids, plants and plant cells expressing anti-sense patatin and

There is disclosed plasmids for the preparation of transgenic plants which are modified through the transfer and the expression of genes localized on the plasmids, that influence the sugar metabolism or the sugar partitioning within a plant. In addition, transgenic plants with modified sugar metabolism or sugar partitioning are described. The genes cause a modified distribution of assimilates in the transgenic plant which result in significant changes in plant habit, such as size, leaf shape, internode separation and root formation, as well as improvements in plant yield. Plasmids and methods are also described which enable foreign proteins to be directed into specific tissues or portions of transgenic plants, including vacuoles, leaves and sink organs.

Plasmids useful for and methods of preparing transgenic plants with

DNA sequences which lead to the formation of levans plasmids containing

There are described 5' transcriptional regulatory regions of plant genes which ensure sink organ specific expression of cis-linked DNA sequences as well as recombinant double stranded DNA molecules comprising these 5' regulatory regions. Furthermore the present invention relates to a method for producing transgenic plants which show a sink tissue specific expression of a desired DNA sequence.

Plant promoters specific for sink organ expression of genes

Expression cassette and plasmids for a guard cell specific expression

Plasmids that comprise a DNA sequence coding for a protein that modifies the metabolism of soluble sugars, for example, an inorganic pyrophosphatase agent and plant cells comprising those plasmids, for the production of transgenic plants that are modified in habit and yield arc described, the plants being modified in habit by the transfer and the expression of genes that intervene in sugar metabolism or sugar distribution inside a plant.

Plasmids for the production of transgenic plants that are modified in

There is disclosed plasmids for the preparation of transgenic plants, as well as the plants, that are modified through the transfer and the expression of genes influencing the sugar metabolism or the sugar partitioning within a plant, which are localised on these plasmids. The transferred genes cause a modified distribution of assimilates in the transgenic plant which result in significant changes in habit, such as size, leaf shape, internode separation and root formation, as well as improvements in yield. Plasmids are also described which enable foreign proteins to be directed into the vacuoles of transgenic plants.

Plasmid for the preparation of transgenic plants with a change in habit

There is described a new DNA sequence of an expression cassette on which the potato tuber specific regulatory regions are localised as well as the transfer of this DNA sequence into the plant genome using agrobacteria as transfer micro-organisms. The DNA sequence contains a patatin gene with a patatin gene promoter. The transfer DNA sequence acts both for regulating endogenous as well as for preparation of heterologous products in crops.

Potato tuber specific transcriptional regulation

The invention relates to wound-stimulated DNA from Solanum tuberosum as well as parts thereof, their use for the development of gene products in higher plants using wounding or pathogen attack, DNA-transfer vectors containing these, and plants or plant portions containing these. The promoter of this DNA-sequence can be used for the expression of gene products in higher plants using wound and/or pathogen attack.

Wound-stimulated DNA-sequence from solanum tuberosum and its use

Growing community of inventors

Berlin, Germany

Lothar Willmitzer