Degussa Aktiengesellschaft

Forschungszentrum Julich Gmbh

Other

Degussa - Huls Ag

Evonik Degussa Gmbh

Forschungszentrum Juelich Gmbh

Evonik Roehm Gmbh

Amino Corporation

Kernforschungsanlage Julich Gesellschaft M.b.h.

Evonik Rohm Gmbh

Lothar Eggeling

Hermann Sahm

Walter Pfefferle

Bettina Mockel

Bettina M&ouml;ckel

Miroslav Patek

Georg Thierbach

Mechthild Rieping

Stephan Hans

Petra Ziegler

Caroline Kreutzer

Michael Bott

Stephan Binder

Petra Peters-Wendisch

Yvonne Tilg

Georg Schendzielorz

Madhavan Nampoothiri K

Achim Marx

Bettina Möckel

Stephanie Bringer-Meyer

Anke Weissenborn

Bernd Eikmanns

Nicole Kennerknecht

Solvej Siedler

Madhavan Nampoothiri

Sahm Hermann

Caroline Kruetzer

Roman Netzer

Brigitte Bathe

Wolfgang Leuchtenberger

Bettina Moeckel

Hermann Siegert

Alexander May

Markus Poetter

Stefan Buchholz

Bernhard Eikmanns

Kevin Burke

Ashling McCormack

Cliona Stapelton

Markus Pötter

Manfred Paschke

Manfred Kircher

Albert De Graaf

Jan Marienhagen

Elisabeth Scheer

Bernd Moritz

Laurence K Dunican

Walter Pfefferie

Marina Vrlijc

Birgit Klassen

Robert Faurie

Birgit Alber

Georg Fuchs

Julia Frunzke

Nurije Mustafi

Hermann Prof Sahm

An NADP(H) nanosensor has i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

Sensor for NADP (H) and development of alcohol dehydrogenases

The present invention relates to an NADP(H) nanosensor comprising The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

A method for producing vectors containing a gene coding for an enzyme having reduced or deactivated feedback inhibition and to the use thereof for producing amino acids and nucleotide. Genes coding for feedback-inhibited enzymes are mutagenized in vitro, &#x2014;ligated into vectors in a further step&#x2014;the vectors are each transformed in a microorganism containing a metabolite sensor in a further step, which brings about the synthesis of a fluorescent protein at an increased metabolite concentration, &#x2014;whereupon microorganisms exhibiting increased or maximum fluorescence are selected; and &#x2014;the vectors that contain a gene coding for an enzyme having reduced or deactivated feedback inhibition are isolated from microorganisms having increased or maximum fluorescence.

Method for producing vectors containing a gene coding for an enzyme having reduced or deactivated feedback inhibition and the use thereof for producing amino acids and nucleotides

The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.

Process for preparing methacrylic acid or methacrylic esters

The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a, especially. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.

Method for the fermentative production of L-valine and suitable microorganism

This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.

Nucleotide sequences of coryneform bacteria coded for proteins participating in L-serine metabolism and method for microbial production of L-serine

L-lysine-producing strains ofwith enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and a process for the preparation of L-lysine.

L-lysine-producing corynebacteria and process for the preparation of L-lysine

The invention relates to nucleotide sequences of coryneform bacteria that encode proteins that are involved in the biosynthesis of L-serine and to a method for producing L-serine. According to the invention, at least 79 amino acids at the C terminus of the wild-type serA sequence are deleted, thereby producing a 3-phosphoglycerate dehydrogenase having a reduced feedback inhibition by L-serine vis-&#xe0;-vis the wild-type sequence.

Nucleotide sequences that encode deregulated phosphoglycerate dehydrogenases of coryneform bacteria and method for producing L-serine

The present invention relates to a process for the microbial production of L-valine in which the dihydroxy acid-synthase (ilvD) activity and/or the ilvD gene expression is intensified in a microorganism. As an alternative or in combination with this, the acetohydroxy acid-synthase (ilvBN) activity and isomeroreductase (ilvC) activity and/or the ilvBNC gene expression are intensified in a microorganism. The process according to the invention preferably makes use of microorganisms in which the activity of at least one enzyme that is involved in a metabolic pathway that reduces the formation of L-valine is weakened or eliminated. Thus, for instance, the process according to the invention preferably makes use of microorganisms having a defect mutation in the threonine dehydratase (ilvA) gene and/or a defect mutation in one or more genes of the pantothenate synthesis.

Method for microbially producing L-valine

The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

L-Lysine-producing corynebacteria and process for the preparation of L-lysine

The present invention relates to nucleotide sequences of coryneform bacteria, coding for proteins involved in the bio-synthesis of L-serine and to methods for the isolation thereof. The invention further relates to an improved method for the production of L-serine. In addition, the present invention relates to the use of L-serine in the food, animal feed and/or pharmaceutical industries or in human medicine.

Nucleotide sequences coding for proteins involved in the biosynthesis of L-serine, an improved method for the microbial production of L-serine and a genetically modified microorganism suitable therefor

The invention relates to a genetically modified coryneform bacterium, the fadD15 gene of which is amplified, and an isolated polynucleotide which codes for acyl-CoA synthase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the fadD15 gene in the bacteria and the use of the polynucleotide as a primer or hybridization probe.

FadD15 gene of Corynebacterium glutamicum, encoding an acyl-CoA synthase polypeptide

The invention relates to preferably recombinant DNA derived fromand replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine, which is characterised in that the following steps are carried out:

Nucleotide sequences coding for the thrE gene and process for the enzymatic production of L-threonine using coryneform bacteria

The invention relates to L-lysine-producing strains of corynebacteria with enhanced pyc gene (pyruvate carboxylase gene), in which strains additional genes, chosen from the group consisting of the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the lysE gene (lysine export carrier gene) and the dapB gene (dihydrodipicolinate reductase gene), but especially the dapA gene, are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

L-lysine-producing corynebacteria and process for the preparation of lysine

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Julich, Germany

Lothar Eggeling