Merck Patent Gesellschaft MIT Beschrankter Haftung

Merck Patent Gmbh

Klawego Gmbh & Co. Kg

Lothar Britsch

Michael Schulte

Egbert Muller

Klaus Gottschall

Jochen Strube

Karl-Heinz Derwenskus

Egbert M&uuml;ller

Margot Mack

Josef-Stefan St&ouml;hr

Evelyn Gottschall

Heiner Graalfs

Method for recovering a target protein from a feedstock comprising said target protein and at least one impurity compound selected from host cell proteins (HCP), DNA, RNA or other nucleic acid, the target protein being characterized by a hydrodynamic radius Rand the impurity compound being characterized by a hydrodynamic radius R, wherein R&#x3e;R, comprising the following steps (i) to (iv) and optionally step (v): (i) providing a polymeric mesh comprising at least one crosslinked polymer containing positively charged amino groups, wherein the polymer has a pore size exclusion limit Rhi which can be set variably; (ii) adapting the variable pore size exclusion limit Rhi of the polymeric mesh such that R&#x3c;Rand R&#x3e;R; (iii) contacting the polymeric mesh with the feedstock; (iv) separating the polymeric mesh containing the retained impurity compound from the feedstock containing the excluded target protein.

Use of a polymeric mesh for the purification of macromolecules

The invention relates to a modified separating material having improved properties, to the preparation thereof, and to the use thereof for the separation of charged biopolymers from liquids.

Graft copolymers for ion exchange chromatography

The invention concerns alkali-stable hydrophilic sorbents for ion-exchange chromatography. The invention is characterized in that it consists in using as base material a crosslinked vinyl polymerconsisting of vinyl monomers containing diol groups and crosslinking monomers whereof the polymerizable groups are bound by carbonamide-containing chains. Monomers containing ionic groups are grafted on said base material using cerium (IV) salts.

Alkali-stable hydrophilic sorbents for ion-exchange chromatography

Continuous separation methods, in partricular using SMB methods, are disclosed, in which the analytes are separated by size exclusion chromatography (gel permeation chromatography).

Continuous method for separating substances according to molecular size

The present invention relates to a process for removing an initiator of cerium (IV) salt from a graft product. The process may include washing the graft product with a mineral acid solution comprising ascorbic acid.

Method for removing initiators from grafting products

Hydroxyl group-containing covalently bound polymer separating materials

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Reute, Germany

Lothar Britsch