Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Biomerieux, Inc.

Loréne Allano

Frédéric Pinston

Dominique Decaux

Corine Fulchiron

Guillaume Sutra

Lorette Munoz

A method for defining an isolation area around an object of interest in a cell culture vessel, the method comprising the steps of: &#x2014;obtaining one or more images of the cell culture vessel using one or more of a plurality of illumination sources, each illumination source being capable of illuminating the vessel from a different direction; &#x2014;selecting an image or combination of images for further processing; &#x2014;applying a circular object detection transformation to identify one or more objects of interest being substantially circular objects in the cell culture vessel, which circular objects of interest are representative of isolated colonies in the cell culture vessel and determining the center of an object of interest; &#x2014;applying a binarizing step to obtain a binarized image of the object of interest and other objects, wherein the center of the binarized image corresponds to the center of the object of interest; &#x2014;iteratively forming concentric circles with increasing radius, wherein the concentric circles are centered on the center of the binarized image; &#x2014;identifying coronas, wherein a corona is delimited by two circles having successive radius values; &#x2014;for each corona: determining the presence and the location of any other object located in the corona to determine the presence and the location of other objects; &#x2014;determining a clearance angle defining an angular sector free of other objects around the object of interest to define the isolation area around the object of interest.

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Sevres, France

Loréne Allano