Bakotic Pathology Associates, LLC

Lori Ilene Bennett

Kiran Madanahally Divakar

Lilly I Kong

Erik Gustafson

Bradley Wayne Bakotic

Idowu Akinsanmi

Rama Murthy Sakamuri

Liquan Yang

Laurie Susan Page

Marianna Agassandian

Provided herein is a real-time PCR-based method of detecting, in a sample, an agent causing onychodystrophy, wherein the agent causing onychodystrophy belongs to a secondary clade member including one or more primary clade members. Also provided are compositions and kits that finds use in implementing the present method.

Methods of screening for causative agents of onychodystrophy

Provided herein is a method of detecting an onychomycotic fungus in a sample, wherein the onychomycotic fungus belongs to a secondary clade member including one or more primary clade members. The method may include the steps of i) screening a sample using a first and second sets of secondary clade-specific primers to determine whether a secondary clade member among a plurality of secondary clade members is present or absent in the sample, where the plurality of secondary clade members includes (a) a dermatophyte, (b) a, and (c) a saprophyte, and ii) after determining the presence of the secondary clade member, screening the sample to determine whether an onychomycotic fungus is present or absent in the sample using primary clade-specific primers that are specific to a primary clade member that belongs to the secondary clade member. Also provided is a kit that finds use in implementing the present method.

Methods of screening for onychomycotic fungi

Growing community of inventors

Alpharetta, GA, United States of America

Lori Ilene Bennett