Butamax Advanced Biofuels LLC

Butamax (Tm) Advanced Biofuels LLC

Inbiose N.v.

Lori Ann Maggio-Hall

Andrew C Eliot

Vasantha Nagarajan

Gail K Donaldson

Dennis Flint

Larry Cameron Anthony

Charles Edwin Nakamura

Steven Cary Rothman

Mark J Nelson

Jean-Francois Tomb

Ranjan Patnaik

Lixuan Lisa Huang

Michael G Bramucci

Hongxian He

Rick Weizhang Ye

Daniel P O'Keefe

Yougen Li

Arthur Leo Kruckeberg

Michael Dauner

Daniel P Okeefe

Jessica McElvain

Brian James Paul

Michael Charles Grady

Wonchul Suh

Lisa L Huang

Mehmedalija Jahic

Lisa A Laffend

Jessica Mcelvain

Katharine J Gibson

Daniel P O'keefe

Charles E Nakamura

Vasantha Nagajaran

A fermentation broth which includes a microbial cell which has been subjected to a condition under which the activity an endogenous transporter for 2' fucosyllactose is increased. Also provided are methods for increasing export of 2′ fucosyllactose from a microbial cell, methods for identifying an endogenous yeast transporter of 2′ fucosyllactose, and microbial cells genetically engineered to increase the activity of an endogenous transporter of 2′ fucosyllactose.

Increasing activity of 2'fucosyllactose transporters endogenous to microbial cells

The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells.

Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion

The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.

Recombinant host cells comprising phosphoketalase

Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.

DHAD variants for butanol production

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

Fermentive production of four carbon alcohols

Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.

Host cells and methods for production of isobutanol

The invention relates to the fields of industrial microbiology and alcohol production. More specifically, the invention relates to improved production of butanol isomers by recombinant microorganisms containing an engineered butanol pathway and disrupted activity of the genes in pathways for the production of by-products during the fermentation when the microorganisms are grown in a fermentation medium containing acetate. In embodiments, recombinant microorganisms have an increased growth rate in a fermentation medium containing acetate as a C2 supplement.

Acetate supplemention of medium for butanologens

The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.

Recombinant host cells comprising phosphoketolases

From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of

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Wilmington, DE, United States of America

Lori Ann Maggio-Hall