University of Chile

Loreto Parra

Luis O Burzio

Juan A Asenjo

Barbara A Andrews

Juan Pablo Acevedo

A method of preparing a cold adapted xylanase by use of recombinant DNA techniques. A nucleic acid and corresponding amino acid sequences of a cold adapted xylanase, isolated from antarctic marine origin, preferably from an Antarctic bacteria (sp.) are provided. These can be used in a variety of industrial contexts and for a variety of commercial purposes including more complete hydrolysis of lignocellulosic biomass into simple sugars that can then be fermented to products, such as liquid fuels and chemical feedstocks. The enzymes are also useful in the production methods of other industries, such as the animal feed, baking, and paper industries. Nucleic acids, corresponding amino acid sequences, constructs, expression vectors or integration vectors containing the DNA molecule, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides for producing and using the-derived cold adapted family GH10 xylanase-like protein are also described.

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Santiago, Chile

Loreto Parra