Boehringer Mannheim Gmbh

Lorenz Kerscher

Joachim Ziegenhorn

Christian Klein

Hans-Georg Batz

Christa Hubner-Parajsz

Ulrich Essig

Wolfgang Rollinger

Brigitte Pautz

Johann Karl

Joachim Siedel

Erich Schneider

Sigbert Schiefer

Gisela Trunk

Immunogens of formula I are provided for obtaining HbA.sub.1c -specific antibodies ##STR1## M is 1 to 40, T is a carrier protein and A is a 10-20 atoms spacer and is ##STR2## where X is S or --NH--, n is 1 to 4 and B is a succinimide containing organic radical. The antibodies obtained bind glycosylated hemoglobin to determine HbA.sub.1c in body fluids.

Immunogens and the use thereof for obtaining antibodies against

The present invention provides immunogens for obtaining HbA.sub.1c -specific antibodies, which immunogens have the general formula: ##STR1## wherein M is 1 to 40, T is a carrier protein and A is a spacer which has a chain length of 10 to 20 atoms and has the general formula: ##STR2## wherein X is a sulphur atom or au --NH-- group, n is 1 to 4 and B is an organic radical which contains a succinimide group. The present invention also provides a process for obtaining antibodies which specifically bind glycosylated haemoglobin, wherein an immunogen of general formula (I) is injected into an organism capable of antibody formation and the antibodies are then obtained in known manner. These antibodies can be used for determining HbA.sub.1c in body fluids.

The invention concerns a method for the determination of the content of a particular haemoglobin derivative in a blood sample. In particular, the invention concerns a method for the determination of the content of haemoglobin derivatives such as glycated haemoglobin which require the separate determination of the total haemoglobin content and the determination of the haemoglobin derivative in order to determine the proportion of the derivatized haemoglobin in the blood as well as a suitable haemolysis reagent for this.

Immunological method for the determination of a haemoglobin derivative

The present invention provides a process for the specific determination of the cholesterol of the HDL fraction in the presence of the LDL fraction of serum lipoproteins. Pancreatic cholesterol esterase is used to liberate cholesterol, and the liberated cholesterol then reacts with cholesterol oxidase and oxygen to form hydrogen peroxide. The kinetics of either of hydrogen peroxide formation or oxygen consumption is measured within 2 to 15 minutes after the start of the reaction between cholesterol and the oxidase. The temperature is maintained within a range of 20.degree. C., during a predetermined time interval. Specific concentrations of reactants are maintained in the reaction solution, i.e., from 0.05 to 30 U/ml pancreatic cholesterol esterase; from 0.1 to 50 U/ml cholesterol oxidase; from 1.0 to 20 mMole/liter of a tenside of the bile acid group, and 0.1 to 10 g/liter of a non-ionic detergent. The pH is kept within a range of 5 to 9. In addition, a reagent is provided which is used for specific determination of HDL fraction cholesterol in the presence of the LDL fraction of serum lipoproteins. The reagent contains pancreatic esterase (0.05 to 30 U/ml); cholesterol oxidase (0.1 to 50 U/ml) a tenside of the bile acid grop (1.5 to 8 mMole/liter) and a non-ionic detergent (0.1 to 10 g/liter), all concentrations referring to the dilution used in the test. The reagent also contains a buffer at a pH of from 5 to 9, and a system for photometric determination of hydrogen peroxide.

Process and reagent for the specific determination of the cholesterol of

The present invention provides a process for the specific determination of HDL cholesterol in serum or plasma by incubation with a cholesterol detection system, containing cholesterol oxidase and cholesterol esterase in a buffered aqueous medium, and measurement of a product of the cholesterol oxidase reaction or of the oxygen consumption, wherein a sample to be tested is incubated in the presence of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate, a first measurement is then carried out, subsequently a non-ionic, polyethylene oxide group-containing detergent or a secondary alkane sulphonate is added, again incubated and a second measurement is carried out, the amount of HDL cholesterol being determined from the difference between the first and second measurement. The present invention also provides a reagent for the specific determination of HDL cholesterol in serum or plasma containing cholesterol oxidase, buffer and cholesterol esterase, wherein, referred to the final solution, it contains

Process and reagent for the specific determination of HDL cholesterol in

A method for determining low density lipoproteins (LDLs) in a body fluid sample, as well as a reagent suited for this use, are taught. The method involves precipitating high density lipoproteins (HDLs) from the sample, using an HDL specific antibody or reactive fragment, and then determining the presence and amount of LDL in the supernatant which results. The reagent contains the HDL specific antibodies, as well as polyanions and divalent cations.

Growing community of inventors

Penzberg, Germany

Lorenz Kerscher