Codexis, Inc.

Lorand Szabo

Louis Clark

Dipnath Baidyaroy

Jie Yang

Onorato Campopiano

Kripa Rao

Janos Torok

Attila Laszlo Nemeth

Zsolt Molnar

The invention provides recombinant GH61 proteins obtained from, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated fromsupernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, &#x3b2;-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.

Use of glycohydrolase 61 protein variants with improved thermostability for processing cellulose

The present invention provides fungal proteases and improved fungal strains that are deficient in protease production.

Fungal proteases

The invention relates to expression of a recombinant C1 &#x3b2;-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting cellobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 &#x3b2;-glucosidase, operably linked to heterologous promoter, under conditions in which &#x3b2;-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said &#x3b2;-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

Recombinant C1 beta-glucosidase for production of sugars from cellulosic biomass

The invention relates to expression of a recombinant C1 &#x3b2;-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting celiobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 &#x3b2;-glucosidase, operably linked to heterologous promoter, under conditions in which &#x3b2;-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said &#x3b2;-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

Growing community of inventors

Budapest, Hungary

Lorand Szabo