Hubei University

Longhai Dai

Chun-Chi Chen

Lilan Zhang

Jian-Wen Huang

Rey-Ting Guo

Jian Min

Yu Yang

Yunyun Yang

Lixin Ma

Xian Li

Chao Zhai

Beilei Shi

Yumei Hu

Panpan Shen

Shan Wu

Wei Zeng

Chin-Yu Chen

Xuejing Yu

Zhichun Zhan

Xiuqin Li

A modified cutinase is disclosed. The cutinase has the modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of asparagine at position 181 with alanine, or substitutions of asparagine at position 181 with alanine and phenylalanine at position 235 with leucine. The modified enzyme has improved PET-hydrolytic activity, and thus, the high-activity PET hydrolase is obtained, and the industrial application value of the PET hydrolase is enhanced.

Cutinase having improved enzymatic activity

A PET hydrolase having improved thermal stability is disclosed. The PET hydrolase has a modified amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence with at least 80% sequence identity of SEQ ID NO: 2, wherein the modification is a substitution of asparagine at position 248 or a corresponding position with proline.

PET hydrolase having improved thermal stability

A cellulase having improved enzymatic activity is disclosed. The cellulase has a modified amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence with at least 80% sequence identity of SEQ ID NO: 2, wherein the modification is a substitution of methionine at position 120 or a corresponding position with asparagine.

Cellulase having improved enzymatic activity

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Wuhan, China

Longhai Dai