Thermo Fisher Scientific Baltics Uab

Vilnius University

Fermentas Uab

Lolita Zaliauskiene

Arunas Lagunavicius

Ausvydas Vareikis

Sarunas Zigmantas

Monika Glemzaite

Giedrius Gasiunas

Tautvydas Karvelis

Arvydas Lubys

Anja Smith

Virginijus Siksnys

Ricardas Makuska

Alma Bockuviene

Laurynas Riauba

Richard Makuska

Ula Bernadisiute

Monika Gasiuniene

Isolation or in vitro assembly of the Cas9-crRNA complex of theCRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit.

RNA-directed DNA cleavage by the Cas9-crRNA complex

Disclosed herein are poly(hydroxylalkyleneimine disulfide)polymers, which have both desirable transfection properties and reduced toxicity.

Biodegradable cationic polymers and uses thereof

A composition comprising a polycationic agent and a polyanionic agent, and kits comprising the composition, is provided. In embodiments, the polyanionic agent is a nucleic acid and the polycationic agent is a modified polyalkyleneimine polymer.

Delivery agent

A composition and method for delivering a peptide or protein into a cell comprising a polycationic agent and a polyanionic agent, wherein the polyanionic agent comprises an inorganic polyphosphate or a polyoxometalate and/or the polycationic agent comprises a polyalkyleneimine.

The present invention relates to a polyalkyleneimine polymer having a repeat unit comprising a hydrophilic group, agents containing the same and methods for their use.

Transfection reagent

A composition and method comprising a polycationic agent and a polyanionic agent for delivering a peptide or protein into a cell.

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Vilnius, Lithuania

Lolita Zaliauskiene