Eic Laboratories, Inc.

Lois S Robblee

A liquid mixture is assayed for a biologically active component that undergoes a reduction or oxidation reaction within a specific voltage range by immobilizing at an electrically conductive surface, a binding partner for the component, exposing the surface to the component to permit complexing to occur, applying a voltage to the surface in a range which will cause the component to undergo an oxidation or reduction reaction, and measuring the resulting current as an indication of the presence of the component.

Assaying for a biologically active component

A metallic electrode made of platinum, platinum iridium alloy, stainless steel, stainless steel alloys, titanium, titanium alloys, tantalum or tantalum alloys is coated with iridium oxide. Ir(III) trichloride is heated in 5.5M HCl until 75-80% of the solution is evaporated. Isopropyl or ethyl alcohol is added to the evaporated solution to restore the solution to its original volume. This restored acid/alcohol solution is aged for 1-2 weeks. The metallic electrode is soaked in the aged acid/alcohol solution for 16 hours, dried for one hour at room temperature and annealed in air at 320.degree. C. for 80-90 minutes.

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Randolph, MA, United States of America

Lois S Robblee