Cetus Corporation

Affymetrix, Inc.

Affymax Technologies, N.v.

Chiron Corporation

Lois Aldwin

Danute E Nitecki

James L Winkler

Christopher J Buchko

Debra A Ross

Douglas N Modlin

Stephen P A Fodor

Stephen P Fodor

Margaret Moreland

David F Mark

Corey H Levenson

Irwin Braude

Henry Rapoport

Nandini Katre

Robert J Goodson

Kirston E Koths

Willem P Stemmer

Walter J Laird

L L Houston

Paula J Shadle

Henry Rapaport

John D Young

Mark Madden

A method and device for forming large arrays of polymers on a substrate (). According to a preferred aspect of the invention, the substrate is contacted by a channel block () having channels () therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodologies.

Combinatorial strategies for polymer synthesis

A method and device for forming large arrays of polymers on a substrate (). According to a preferred aspect of the invention, the substrate is contacted by a channel block () having channels () therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.

A method for producing arrays by spacing a dispenser a distance from a surface of a support, dispensing a volume containing a compound in a single coupling step of less than 5 nl to occupy a localized area of less than 1 cmof the surface of the support, allowing the compound to bind directly or indirectly to the support and repeating the steps to produce an array of at least 100 ligands at a density of 1000 per cmor greater.

A method for producing polymer arrays by spacing a dispenser a distance from a surface of a support, dispensing a volume containing a monomer in a single coupling step of less than 5 nl to occupy a localized area of less than 1 cmof the surface of the support, allowing the monomer to bind directly or indirectly to the support and repeating the steps to produce an array of at least 100 polymer ligands at a density of 1000 per cmor greater.

A method for producing arrays by depositing a resist on a substrate, selectively removing a portion of the resist to expose localized areas, dispensing a monomer to occupy a localized area of less than 1 cmof the surface of the support, allowing the monomer to bind directly or indirectly to the support and repeating the steps to produce an array of at least 10 different polymers is formed.

A device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.

Combinatorial kit for polymer synthesis

An array is created by moving a dispenser toward a solid support until a tip of the dispenser touches the support, withdrawing the tip from the surface and releasing a drop of 5 nanoliters or less in an area smaller than 1 mm.sup.2 to create an array of at least 100 spots.

A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are flowed through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.

Very large scale immobilized polymer synthesis using mechanically

A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.

A coupling agent which is an activated ester such as N-maleimido-6-aminocaproyl-HNSA (mal-sac-HNSA) is formed by reacting 4-hydroxyl-3-nitrobenzene sulfonic acid sodium salt (HNSA) with a carboxylic acid moiety of a compound such as N-maleimido-6-aminocaproic acid. The coupling agent is reacted with an amino group of an amine-containing biological material such as a protein at a pH of about 5.5 to 10.0 and HNSA is released. The released HNSA is spectroscopically measured at a wavelength of from about 350 nm to about 500 nm to precisely monitor and control conjugating of the coupling agent to the biological material. The resulting product is coupled to a sulfhydryl group or other group of another material to provide cross-linking between the two. This enables joining the biological material to one another, to a support matrix, to a label, to a hapten, and to other materials. An immunotoxin for therapeutic use can be prepared by conjugating the coupling agent to an antibody such as a monoclonal antibody with tumor cell specificity and then joining the resultant conjugate to a cytotoxic molecule.

Method of conjugating an activated ester to an amine-containing

Peptides that form lightly associated homodimers can be used to form dimers and multimers of other molecules and molecular motifs of interest. These association peptides can dimerize regardless of whether motifs are added to the amino-terminus of the peptide, or the carboxy terminus of the peptide, although additions to the carboxy-terminus of the association peptides require the presence of certain acidic residues.

Association peptides

Succinylacetone derived or related medicaments and methods of synthesis of the same are shown wherein the medicaments consists of succinylacetonyl-proline-PEG, succinylacetonyl-NH-PEG, or compounds that have the formula: ##STR1## wherein n=1-6

Immunosuppressive analogues and derivatives of succinylacetone

Succinylacetone derived or related medicaments and methods of synthesis of the same are shown wherein the medicaments consists of succinylacetonyl-proline-PEG, succinylacetonyl-NH-PEG, or compounds that have the formula: ##STR1## and that have immunosuppressive activity both in vivo and in vitro based on their activities in cellular immunologic assays and adjuvant induced arthritis in rats, respectively.

Growing community of inventors

San Mateo, CA, United States of America

Lois Aldwin