Csir

Northwest University

Potchefstroom University for Christian Higher Education

Lekratek Instrumentation

Lodewyk Jacobus Mienie

Ronald A Van Steenderen

Christoffel Petrus Stephanus Badenhorst

Henning J Els

Wouter J De Wet

Julius H Ueckermann

Alberdina Aike Van Dijk

Rencia Van Der Sluis

Elardus Erasmus

This invention relates to a method of producing a recombinant enzyme, more particularly, this invention relates to a method of producing water soluble enzymatically active recombinant glycine N-acyltransferase (GLYAT (E.G. 2.1.3.13)), including the steps of providing a suitable expression host; preparing a vector including a gene for expressing GLYAT in the expression host to form an expression plasmid; transforming the host with the expression plasmid to form an expression system; expressing the GLYAT gene in the expression system; and separating the expressed GLYAT from the expression system.

Recombinant therapeutic glycine N-acyltransferase

A method and apparatus for the preliminary detection of abnormal levels of metabolites in the urine of a human or mammal. The method includes the steps of determining the amount of creatinine in the urine of the human or animal, measuring the total metabolites content in the urine, using the amount of creatinine in the urine as an internal standard to compensate for variations in the urine concentration, and comparing the total metabolites content in the urine with a reference value which corresponds to the expected total metabolites content in the urine of a person or animal who does not suffer from a metabolic disorder. The apparatus (10) includes an ultraviolet oxidation unit (16) for oxidising total metabolites in a urine sample to carbon dioxide, an organic carbon stripper (18) for stripping carbon dioxide gas from the oxidised sample, and a carbon dioxide detector (69) for measuring the amount of carbon dioxide produced from the sample.

Fluid analysis

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Potchefstroom, South Africa

Lodewyk Jacobus Mienie