Centre National De La Recherche Scientifique

Institut National De La Sante Et De La Recherche Medicale

Institut Pasteur

Aventis Pharma S.a.

Assistance Publique-hopitaux De Paris

Institut Gustave Roussy

Université Pierre Et Marie Curie

Lluis M Mir

Daniel Scherman

Philippe Kourilsky

Claude Roth

Michel Bureau

David Klatzmann

Jean-Loup Salzmann

Stephane Orlowski

Michel Siros

The invention provides a method of transferring in vivo a molecule into a striated muscle cell. More specifically, a method of the invention comprises contacting in vivo a striated muscle cell with a molecule, and electrically stimulating the muscle cell with one or more unipolar pulses of an electric field intensity ranging from 1 to 800 V/cm. In one embodiment, the molecule is a nucleic acid encoding a protein of interest. For example, the invention provides methods of promoting angiogenesis and hemostasis.

Method for transferring nucleic acid into striated muscles

The invention concerns an improved method for transferring in vivo multicelled eukaryotic organism cells nucleic acids or nucleic acids combined with products for enhancing the efficacy of such transfers. The invention also concerns the combination of a nucleic acid and the transfer method for use in gene therapy.

Method for transferring nucleic acid into multicelled eukaryotic organism cells and combination therefor

Composition for use in the treatment of tumors and the immunization of humans or animals comprising a synergistic association of cells, viruses, or bacteria expressing, transitorily, in organisms at least one gene for producing in vivo one or more immunomodulators, and viruses, or cells producing viruses, said viruses preferably infecting dividing cells of the treated organisms and carrying within their genome at least one gene whose expression in the dividing cells will cause their destruction.

Composition comprising interleukin-2 and thymidine kinase for the

Composition designed to treat human or animal organisms comprising cells expressing genes enabling them to secrete in vivo one or more biologically-active substances, said cells exhibiting genetic characteristics preventing them from growing durably in the treated organism, and making them susceptible to elimination artificially or naturally from the organism. These compositions can be used in particular in the treatment of tumors or cancers, in which case the substances used are interleukins. The cells contained in these compositions are at least partially allogenic or xenogenic.

Cellular composition for the treatment of human or animal organisms

Electric pulse applicator using pairs of needle electrodes for the

Growing community of inventors

Verrieres le Buisson, France

Lluis M Mir