Gen-Probe, Inc.

Lizhong Dai

Norman C Nelson

Lyle J Arnold, Jr

Jijumon Chelliserry

Steven Scott Phelps

Daniel L Kacian

James Russell

Kenneth A Browne

Margarita B Kaminsky

Mark E Filipowsky

Mark E Flipowsky

Nornan C Nelson

Daniel B Kacian

Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

Multiphase nucleic acid amplification

The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.

Solutions, methods and kits for deactivating nucleic acids

Method of making a formulation for deactivating nucleic acids

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San Diego, CA, United States of America

Lizhong Dai