Integrated Dna Technologies, Inc.

Liyang Zhang

Christopher Anthony Vakulskas

Mark Aaron Behlke

Michael Allen Collingwood

Nicole Mary Bode

Kristin Beltz

Nathaniel Hunter Roberts

The present disclosure concerns polynucleotides and amino acids of. Cas12a (Cpf1) and methods for their use for genome editing in eukaryotic cells.

Methods using novel CPF1 mutations that enhance the DNA cleavage activity ofsp. CPF1

Described herein are CAS12A mutants from Lachnospiraceae bacterium and methods for use thereof. These mutants have enhanced DNA cleavage activities at non-canonical TTTT protospacer adjacent motifs (PAM) compared to the wild-type enzyme.

sp. Cas12a mutants with enhanced cleavage activity at non-canonical TTTT protospacer adjacent motifs

This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays increased on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRISPR/Cas endonuclease systems having mutant Cas9 proteins that display increased on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.

Mutations inCas9 discovered by broad scanning mutagenesis demonstrate enhancement of DNA cleavage activity

Described herein are CAS12A mutants fromand methods for use thereof. These mutants have enhanced DNA cleavage activities at non-canonical TTTT protospacer adjacent motifs (PAM) compared to the wild-type enzyme.

sp. CAS12A mutants with enhanced cleavage activity at non-canonical TTTT protospacer adjacent motifs

The present disclosure concerns polynucleotides and amino acids ofsp. Cas12a (Cpf1) and methods for their use for genome editing in eukaryotic cells.

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Coralville, IA, United States of America

Liyang Zhang