Roche Sequencing Solutions, Inc.

Genia Technologies, Inc.

Liv Elisabeth Jensen

Christos Tzitzilonis

Cynthia Ann Cech

Corissa Harris

Matthew Dipietro

Dhruti Vasudev Dalal

Charlotte Yang

Tim Craig

Alexander Yang

Michael Dorwart

Timothy Kellogg Craig

Marshall Winston Porter

Alexander Hyun-min Yang

Cynthia Cech

The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

OmpG variants

OMPG variants

Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

Alpha-hemolysin variants

Growing community of inventors

Palo Alto, CA, United States of America

Liv Elisabeth Jensen