Jiangsu Academy of Agricultural Sciences

Liting Hou

Jibo Hou

Xuwen Qiao

Jin Chen

Qisheng Zheng

Xiaoming Yu

Yuanpeng Zhang

Pengcheng Li

Yiwei Wang

The present invention relates to the preparation of the compound immunopotentiator and the application thereof in a foot-and-mouth disease vaccine of pigs. According to the present invention, the foot-and-mouth disease vaccine of pigs is taken as a research subject, and on this basis, several immunopotentiators having obvious immunopotentiating effects are selected for the compound immunopotentiator, and an antigen/vaccine is mixed with the immunopotentiator to prepare a vaccine-immunized pig. An animal experiment result shows that the present invention has obvious immunopotentiating effects. After immunizing the vaccine containing the compound immunopotentiator, a window period for antibody production can be significantly shortened to 7 days; a LPB-ELISA antibody titer is significantly improved, and an antibody pass rate is significantly increased; an immune protection period is also significantly extended, at least up to 7 months.

Immunopotentiator, foot-and-mouth disease inactivated vaccine and preparation method thereof

A method for inactivated foot-and-mouth disease virus antigen purification and concentration, which relates to the field of biotechnology which includes the following steps of: (1) adding an adaptor protein with an amino acid sequence as shown in SEQ ID NO: 2 into the inactivated foot-and-mouth disease virus antigen, mixing evenly, and incubating; (2) adding a purified carrier, mixing evenly, and incubating the mixture, the purified carrier being askeleton; and (3) centrifuging and obtaining a precipitate.

Method for purifying and concentrating inactivated antigen of foot-and-mouth disease virus

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Nanjing, China

Liting Hou