Verax Biomedical, Inc.

Cambridge Biotech Corporation

First Light Biosciences, Inc.

Straus Holdings Inc.

Lisa Shinefeld

Adam P Lousararian

Yli Remo Vallejo

Gregory M Lawrence

Sadanand Gite

Don Straus

Ezra Solomon Abrams

Gordon Siek

Greg Yantz

Bruce P Neri

Sara Bauminger

Obsidiana Abril

Daniel E Cox

Ezra Abrams

The present disclosure provides methods and lateral flow devices for detecting a plurality of target analytes in a liquid sample. In some implementations, the disclosed lateral flow device comprises a housing unit, a capillary flow bed, a sample-receiving zone, a buffer-receiving zone, and a capture zone. The device is configured to control the flow of the sample and reagent buffer in a sequential manner with minimal mixing. In some implementations, the disclosed method is capable of detecting a plurality of target analytes in an assay by applying the binding agents and the signaling agents in separate or sequential steps.

Sequential lateral flow device

The invention provides an improved method for sensitive and specific detection of target molecules, cells, or viruses. The inventive method uses large area imaging to detect individual labeled targets complexed with a target-specific selection moiety. The invention eliminates wash steps through the use of target-specific selection through one or more liquid layers that can contain optical dye and density agents. By eliminating washes the invention simplifies instrumentation engineering and minimizes user steps and costs. The invention uses sensitive image analysis to enumerate individual targets in a large area, is scalable, and can be deployed in systems ranging in complexity from manual to highly automated.

Method for detecting analytes

A capillary flow device useful in double capture assays, such as double capture immunoassays, and a double capture assay. The capillary flow device comprises a capillary track, a sample receptacle, a particle collecting means and, optionally, a magnet and a particle concentrator. The assay method is useful for determining any analyte of interest for which there is a specific binding partner.

Double capture assay method employing a capillary flow device

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Lexington, MA, United States of America

Lisa Shinefeld