Janssen Pharmaceutica Nv

Lisa Minor

Patricia Andrade-Gordon

Raymond W Colburn

Bruce P Damiano

Yuanping Wang

Dennis Stone

Jenson Qi

Jianshen Qi

Robert Schulingkamp

Christopher Van Besien

Administration of U-II to rats caused an increase in the redness or the skin temperature of the ear of the rats. The increase was inhibited by compounds that decease the biological activity of the U-II/UT receptor. Thus, the present invention provides methods of measuring the biological activity of an U-II receptor by monitoring the redness or the skin temperature of the ear of an animal, and the use of the methods to test compounds for their abilities to increase or decrease the biological activity of an U-II receptor.

Method of measuring the biological activity of an urotensin II receptor

Human cell clones with increased specific binding to urotensin II have been sub-cloned from SJRH30 (ATCC&#xae; Number: CRL-2061&#x2122;). These cell clones have been used for functional analyses of the biological activity of an endogeneous urotensin II receptor, as well as for identifying compounds that regulate the biological activity of an Urotensin II receptor.

Human cell clones having an endogeneous urotensin II receptor

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Flemington, NJ, United States of America

Lisa Minor