University of Michigan

Stel Technologies, LLC

Lisa M Larkin

Ellen M Arruda

Michael J Smietana

Pablo Moncada-Larrotiz

Asheesh Bedi

Fatima N Syed-Picard

Jinjin Ma

Stoyna Novakova

Sarah C Calve

Jennifer Baltich

Aaron Adams

Leah Hatch-Vallier

Tatiana Y Kostriminova

Pablo Moncado-larrotiz

Haley Titinger

Michael Smietana

A bioreactor capable of producing complex, three-dimensional tissue constructs has improved media transfer and increased controllability with regard to exposure to the external environment. The bioreactor includes an external surface, a first tissue culture side for culturing a first cell source in a first tissue culture support region, a second tissue culture side for culturing a second cell source in a second tissue culture support region, and a plurality of ports. At least one of the ports of the plurality of ports extend from the first tissue culture support region of the first tissue culture side to the external surface. The plurality of ports can include an external port that is configured to be a liquid inlet when the bioreactor is in a first orientation and a gas outlet when the bioreactor is in a second orientation.

Bioreactor assembly, bioreactor, and method of operating same

The present disclosure relates to tissue engineering, and more particularly to a method for treating or repairing rotator cuff or other tendon tears or damage using scaffold-free, 3-dimensional engineered tendon constructs.

Engineered tendon graft for rotator cuff repair

A bioreactor and method of forming complex three-dimensional tissue constructs in a single culture chamber. The bioreactor and methods may be used to form multi-phasic tissue constructs having tissue formed from multiple cell types in a single culture chamber. The bioreactor includes at least one translation mechanism to facilitate translation of one or more tissue constructs without direct user intervention, thereby providing a closed, sterile environment for complex tissue fabrication. The bioreactor may be used as a stand-alone device or as part of a large-scale system including many bioreactors. The large-scale system may include a perfusion system to monitor and regulate the tissue culture environment.

Bioreactor and method of forming complex three-dimensional tissue constructs

The present disclosure relates to tissue engineering, and more particularly to a method for treating or repairing rotator cuff or other tendon tears or damage using scaffold-free 3-dimensional engineered tendon constructs.

The invention relates to scaffold-free three dimensional nerve fibroblast constructs and method of generating the nerve fibroblast constructs. The invention also relates to methods or repairing nerve transection and replacing damaged nerve tissue using the nerve fibroblast constructs of the invention.

Scaffold-free three dimensional nerve fibroblast constructs

A system and method for forming a bone construct include providing bone marrow stromal cells on a substrate without disposing the cells within an exogenous scaffold, and culturing the cells in vitro in osteogenic media such that the cells form a confluent monolayer and detach from the substrate to form a self-organized three-dimensional bone construct. A system and method for forming a ligament construct using fibrogenic media and a system and method for forming a functionally integrated bone-ligament construct are also provided.

System and method for forming bone, ligament, and bone-ligament constructs

A system and method for forming a skeletal muscle construct include primary muscle cells provided on a substrate without disposing the cells within an exogenous scaffold, the cells cultured in vitro such that the cells form a confluent monolayer; at least two anchors secured to the monolayer in spaced relationship; and at least one secondary tissue, such as neural or tendon tissue, provided in contact with the monolayer such that the monolayer detaches from the substrate and self-organizes to at least partially surround the at least one secondary tissue, thereby forming a three-dimensional skeletal muscle construct having a functional interface with the secondary tissue.

Growing community of inventors

Ann Arbor, MI, United States of America

Lisa M Larkin