E.i. Dupont De Nemours and Company

Other

Butamax Advanced Biofuels LLC

Lisa L Huang

Stephen M Allen

Saverio Carl Falco

J Antoni Rafalski

Vasantha Nagarajan

Lori Ann Maggio-Hall

Daniel P O'Keefe

Andrew C Eliot

Arie Ben-Bassat

Hongxian He

Yougen Li

Sharon Loretta Haynie

Larry Cameron Anthony

Mark J Nelson

Arthur Leo Kruckeberg

David J Lowe

Gail K Donaldson

Steven Cary Rothman

Tanja Maria Gruber

Fateme Sima Sariaslani

Ranjan Patnaik

Jessica McElvain

Jessica Mcelvain

Katharine J Gibson

Daniel P O'keefe

Methods for the microbial production of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) are provided. Microbes producing either tyrosine or phenylalanine are grown in the presence of either tyrosine ammonium lyase or phenylalanine ammonium lyase respectively where some part of the fermentation is accomplished at alkaline pH. The process results in greater yields and higher rates of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) production as compared with fermentation exclusively at physiological pH.

Methods for the preparation of para-hydroxycinnamic acid and cinnamic acid at alkaline pH

An L-arabinose inducible expression system comprising a mutant arabinose promoter. This system exhibits an increase in heterologous protein production upon induction with L-arabinose and comprises a mutant araB promoter and an AraC transcription binding region. This system retains the tight regulatory control characteristic of the wild type arabinose operon.

Mutant arabinose promoter for inducible gene expression

A biocatalytic method for preparing para-hydroxystyrene from para-hydroxycinnamic acid is described. The method uses an enzyme source having para-hydroxycinnamic acid decarboxylase activity to catalyze the decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium to produce para-hydroxystyrene, which is extracted into the organic phase of the biphasic reaction medium. The method results in a high yield of para-hydroxystyrene due to the decreased exposure of the enzyme source to the inhibitory product. The product is readily recovered from the extractant, or may be chemically derivatized directly in the extractant before recovery.

Method for preparing para-hydroxystyrene by biocatalytic decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium

This invention relates to an isolated nucleic acid fragment encoding a histidine biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the histidine biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the histidine biosynthetic enzyme in a transformed host cell.

Plant glutamine amidotransferase homologs

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Hockessin, DE, United States of America

Lisa L Huang