Ventana Medical Systems, Inc.

Roche Sequencing Solutions, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Lisa L Gallegos

Nelson Alexander

Aoune Barhoumi

Stacey Stanislaw

Jigar Patel

Jan Berka

Sedide Ozturk

Esteban Roberts

Seoyoung Kim

Rui Chen

Florian Rubelt

Toumy Guettouche

Maeve E O'Huallachain

Toummy Guettouche

Samantha Rajkovich

Melinda Day

Eric Walk

Katherine Leith

Nelson R Alexander

Maeve E O'HUALLACHAIN

Disclosed herein is a method of analyzing flow cytometry data for cells derived from homogenized whole tumor samples.

Size-based gating to analyze flow cytometry data

The disclosure generally relates to the preparation of representative samples from clinical samples, e.g., tumors (whole or in part), lymph nodes, metastases, cysts, polyps, or a combination or portion thereof, using mechanical and/or biochemical dissociation methods to homogenize intact samples or large portions thereof. The resulting homogenate provides the ability to obtain a correct representative sample despite spatial heterogeneity within the sample, increasing detection likelihood of low prevalence subclones, and is suitable for use in various diagnostic assays as well as the production of therapeutics, especially 'personalized' anti-tumor vaccines or immune cell based therapies.

Representative diagnostics

The present disclosure provides a method of separating cellular particles from a tissue sample and then sorting the cellular particles into two or more cellular particle populations.

Size-based separation of dissociated fixed tissues

The invention is a method of identifying a cognate antigen for a T-cell receptor using neoantigens from a patient's tumor cells combined with the patient's T-cells and using cell sorting, genome sequencing, expressing TCR genes, presenting tumor neoantigens on MHC complex and uniquely barcoding the T-cells where TCR recognition occurs to tag all components of the TCR recognition complex.

System for identification of antigens recognized by T cell receptors expressed on tumor infiltrating lymphocytes

Growing community of inventors

Tucson, AZ, United States of America

Lisa L Gallegos