General Electric Company

Global Life Sciences Solutions USA LLC

Lisa Anne Lowery

Anup Sood

James Edward Rothman

Hae Won Lim

John Donald Burczak

Praveena Mohan

John Richard Nelson

Robert Scott Duthie

Patrick McCoy Spooner

John Anthony Schiel

Anja Josifa Smith

Patrick Mccoy Spooner

A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5' flanking sequence and a donor 3&#x2032; flanking sequence. The donor 5&#x2032; flanking sequence and the donor 3&#x2032; flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.

Site-specific DNA modification using a donor DNA repair template having tandem repeat sequences

The present invention relates to a microbubble complex comprising a microbubble having an outer shell comprising a mixture of native and denatured albumin encapsulating a perfluorocarbon gas, a therapeutic agent, a bifunctional linker having one end attached to the therapeutic agent and the other attached to a ligand and wherein the ligand is bound to the other shell of the microbubble through hydrophobic interactions. Also included are methods for delivering the aforementioned microbubble complex to a tissue target.

Microbubble complexes and methods of use

Growing community of inventors

Niskayuna, NY, United States of America

Lisa Anne Lowery