Basf Plant Science Gmbh

Liqun Xing

Hee-Sook Song

Hongmei Jia

Jeffrey A Brown

Lesley Ireland

Linda Patricia Loyall

Michael Kock

John McMillan

Paula Olhoft

Huihua Fu

Allan R Wenck

Luke Mankin

Marc Morra

Larry Nea

Dwight-Steven Hill

Effie Toren

Christian Dammann

Allan Richard Wenck

John Mcmillan

The invention relates to efficient, high-throughput methods, systems, and DNA constructs for identification and isolation of transcription termination sequences. The invention relates further to specific terminator sequences identified by said methods isolated from rice.

Method for isolation of transcription termination sequences

The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.

Expression enhancing intron sequences

The present invention relates 'disarmed' strain variants of Agrobacterium strain K599 (NCPPB 2659), 'disarmed' plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.

Disarmedstrains, ri-plasmids, and methods of transformation based thereon

The invention relates to efficient, high-throughput methods, systems, and DNA constructs for identification and isolation of transcription termination sequences. The invention relates further to specific terminator sequences identified by the methods isolated from rice.

The present invention relates 'disarmed' strain variants ofstrain K599 (NCPPB 2659), 'disarmed' plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.

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Chapel Hill, NC, United States of America

Liqun Xing