Hoboomlife Bio-technology (shenzhen) Co., Ltd.

Shenzhen University

Liqing Zhao

Jiansheng Chen

Haichao Zhang

Beijia Huang

Zhigang Duan

Muhammad Shahid Riza Rajoka

The present disclosure provides an innovative method for improving the enzyme activity of an NMN biosynthetic enzyme Nampt, and relates to the technical field of genetic engineering. A mutant protein of the present disclosure is obtained by firstly analyzing a target protein Nampt using two softwares FoldX and DeepDDG, and then predicting multiple key sites influencing the enzyme functions and finally performing the semi-rational design of the enzyme. In the examples of the present disclosure, 10 mutant strains are constructed using the designed primers according to the principle of point mutation, and 8 of the mutants have higher activity than a wild-type strain, in which the NMN yield of the mutant Nampt-V365L is increased by 62%, and the NMN yields of the mutants Nampt-S248A, Nampt-N164L, Nampt-S382M, Nampt-A245T and Nampt-A208G are increased by 34%, 27%, 27%, 22% and 17% respectively.

Innovative method for improving enzyme activity of NMN biosynthetic enzyme nampt

Provided isMSR101, having the accession member being CGMCC No. 17506. The strain has acid resistance, bile salt resistance, resistance to phenol, antibiotic resistance, antioxidant activity, cell surface hydrophobicity, adhesion to intestinal epithelial cells, and a cholesterol-lowering function.

Strain of caucasus yoghurtMSR101 and use thereof

Innovative method for improving enzyme activity of NMN biosynthetic enzyme Nampt

Growing community of inventors

Guangdong, China

Liqing Zhao