California Institute of Technology

Lior S Pachter

Jase Gehring

Jong Hwee Park

Akshay Tambe

Siyu Chen

Taleen Dilanyan

Eduardo Da Veiga Beltrame

Disclosed herein include methods, compositions, and systems for extending particle-associated oligonucleotides. In some embodiments, the methods of extending particle-associated oligonucleotides enable efficient methods of preparing a library of barcoded beads. It is also provided, in some embodiments, hydrogel beads degradable upon application of a chemical stimulus that comprise releasably attached oligonucleotides.

Efficient combinatorial bead barcoding

Disclosed herein include systems, methods, compositions, and kits for multiplexing single-cell RNA-sequencing (scRNA-seq) samples. In some embodiments, the methods comprise chemically tagging cells with identifying sample tags (e.g., barcoded DNA oligonucleotides).

Sample multiplexing for single-cell RNA sequencing

Growing community of inventors

Pasadena, CA, United States of America

Lior S Pachter