Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Commissariat a L'energie Atomique

Iprasense Sas

Horiba Abx Sas

Glaxosmithkline Intellectual Property Development Limited

Lionel Herve

Cedric Allier

Jean-Marc Dinten

Anne Koenig

Cédric Allier

Pierre Blandin

Olivier Cioni

Geoffrey Esteban

Thomas Bordy

Pierre Joly

Christine Robert-Coutant

Sophie Morel

Anais Ali Cherif

Ludovic Lecordier

Anabela Da Silva

Damien Isebe

Laurent Guyon

Martin Pisaneschi

Dorothée Krämer

Melissa Hill

The invention describes an iterative reconstructing method allowing a spatial distribution of fluorescence in an object to be obtained. The method comprises acquiring images of fluorescence in various planes at various depths in the object, so as to form a three-dimensional acquired image. It comprises an iterative reconstructing algorithm with, in each iteration, an initial fluorescence distribution or a fluorescence distribution resulting from a preceding iteration being taken into account, and the fluorescence light wave propagating through the object being simulated, so as to obtain a reconstruction of the acquired image. The acquired image, or a differential image corresponding to a comparison between the acquired image and the reconstructed image, is then back-propagated through the object, so as to update the fluorescence distribution. FIG.B.

Method for estimating a three-dimensional spatial distribution of fluorescence, inside an object

A method for determining a state of a cell, the cell being placed in a sample in contact with a culture medium, includes illuminating the sample with a light source and acquiring an image of the sample with an image sensor, the image sensor lying in a detection plane. From the acquired image, a position of the cell in a plane parallel to the detection plane is located and a refractive index or a relative refractive index of the cell is estimated, the relative refractive index corresponding to a refractive index of the cell relative to the refractive index of the culture medium. From the estimation, an index of interest of the cell is determined. From the index, a state of the cell among predetermined states is classified, the predetermined states including at least one apoptosis state and one living state.

Method for determining the viability of cells

Method for detecting or predicting the occurrence of a cell event, selected from between cell division or cell death, in a sample (), the sample extending in at least one sample plane (P) and comprising cells () immersed in a medium, the method comprising the following steps:

Method for detecting a cell event

A method for characterizing a particle present in a sample, the sample lying between an image sensor and a light source and the sensor lying in a detection plane, includes illuminating the sample with the light source which emits an incident light wave propagating along a propagation axis, and acquiring an image of the sample with the sensor. The sensor is exposed to an exposure light wave. The image includes a plurality of elementary diffraction patterns each corresponding to one particle. The method also includes reconstructing a complex image representative of a complex amplitude of the light wave on a reconstruction surface passing through the sample, based on the acquired image; selecting a region of interest of the complex image corresponding to a particle of interest; forming an extracted image based on the region of interest; and characterizing the particle of interest depending on the extracted region of interest.

Method for characterising a particle on the basis of a hologram

A method for observing a sample (), the sample lying in a plane of the sample defining radial coordinates, the method comprising the following steps:

Method for observing a sample

A method for observing a sample, the sample lying in a sample plane defining radial positions, parameters of the sample being defined at each radial position, the method comprising:

Holographic reconstruction method

A method for determining a parameter of a particle present in a sample, the method comprising the following steps: The particle may associated with a set of parameters, comprising at least a size of the particle and a refractive index of the particle.

Method for determining parameters of a particle

A method for holographic characterization of a particle contained in a sample, based on an image, or hologram, of the sample obtained by an image sensor when the sample is illuminated by a light source. The hologram is the subject of a holographic reconstruction, to obtain a reference complex image, representative of the light wave transmitted by the sample in a reconstruction plane. A holographic propagation operator is applied to the reference complex image, to obtain a plurality of secondary complex images, from which a profile is determined describing the change in an optical feature of the light wave transmuted by the sample along the axis of propagation of the light wave.

Holographic method for characterizing a particle in a sample

The invention relates to a method for observing a sample (), comprising the illumination of the sample using a light source () and the acquisition of an image (Io) of the sample using an image sensor (), the sample being disposed between the image sensor and the light source. Iterative steps are applied to the acquired image (Io), also referred to as a hologram, comprising: a single iterative numerical propagation (h), such as to estimate a complex image (A) of the sample in a reconstruction plane (P) or in a detection plane (P), in which the image sensor extends. The complex image can be used for the characterisation of the sample.

Method for observing a sample, by calculation of a complex image

There is provided a device allowing a sample to be observed in a first mode, by lensless imaging using a first sensor. The first mode allows a first image to be obtained, on the basis of which a region of interest of the sample may be identified. The device then allows, via a relative movement, the region of interest to be analyzed using a more precise second mode and in particular using an optical system coupled to a second sensor.

Device for observing a sample and method for observing a sample

A method for observing a sample includes illuminating the sample with a light source and forming a plurality of images, by an imager, the images representing the light transmitted by the sample in different spectral bands. From each image, a complex amplitude representative of the light wave transmitted by the sample is determined in a determined spectral band. The method further includes backpropagation of each complex amplitude in a plane passing through the sample, determining a weighting function from the back-propagated complex amplitudes, propagating the weighting function in a plane along which the matrix photodetector extends, updating each complex amplitude, in the plane of the sample, according to the weighting function propagated.

A method for identifying a state of a cell contained in a sample, including: illuminating the sample using a light source by producing an incident light wave propagating toward the sample; then acquiring, using a matrix-array photodetector, an image of the sample, the sample being placed between the light source and the matrix-array photodetector such that the matrix-array photodetector is exposed to a light wave resulting from interference between the incident light wave and a diffraction wave produced by each cell; applying a numerical reconstruction algorithm to the image acquired by the matrix-array photodetector, to estimate a characteristic quantity of the light wave reaching the matrix-array detector, at a plurality of distances from the matrix-array photodetector. The value of the characteristic quantity, or its variation as a function of distance, allows the state of the cell to be determined from among predetermined states.

Method for determining the state of a cell

A method for identifying a particle contained in a sample, including illuminating the sample using a light source, the light source producing an incident light wave propagating toward the sample, then acquiring, using a matrix-array photodetector, an image of the sample, the sample being placed between the light source and the photodetector such that the matrix-array photodetector is exposed to a light wave that is the result of interference between the incident light wave and a diffraction wave produced by each particle. The method further includes applying a numerical reconstruction algorithm to the image acquired by the photodetector, to estimate a characteristic quantity of the light wave reaching the detector, at a plurality of distances from the detector. The variation in the characteristic quantity as a function of distance allows the particle to be identified.

Method for identifying blood particles using a photodetector

A method reconstructing optical properties of a medium uses a reconstruction system having a radiation source illuminating a medium and a detector receiving a signal emitted by the medium. This method includes establishing, for a source-detector pair, a first distribution of a signal received by the detector for a reference medium, the received signal emitted by the reference medium subsequent to the illumination of the medium by the source, determining, for the source-detector pair, a first modeling function of a light scattering signal between the source and the detector in the reference medium, establishing, for the source-detector pair, a second distribution of a signal received by the detector for the medium, the received signal being emitted by the medium to be characterized subsequent to illumination of said medium by the source, and computing a signal corrected as a function of the first modeling function and of the second distribution.

Method for reconstructing the optical properties of a medium with computing of a signal corrected as a function of a first modeling function for a reference medium and of a second distribution for a medium to be characterized, and associated reconstruction system

A method reconstructing the optical properties of a medium using a reconstruction system has a radiation source lighting the medium and a detector receiving a signal transmitted by the medium. The steps include lighting the medium using a radiation source, receiving by the detector of a signal emitted by the medium, and processing, for a source-detector pair, of a first distribution of the signal received by the corresponding detector. Then computing the Mellin-Laplace transform, for a given order and a given variable, of a magnitude comprising the first distribution, the order being an integer, the variable being a real number, and reconstructing optical properties of the medium using the Mellin-Laplace transform of said magnitude. The computation step includes computing a plurality of Mellin-Laplace transforms of the magnitude for distinct values of the order, and the reconstruction step is carried out from a combination of the plurality of Mellin-Laplace transforms.

Method for reconstructing the optical properties of a medium using a combination of a plurality of mellin-laplace transforms of a magnitude comprising a time distribution of a received signal, and associated reconstruction system

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