Anhui Agricultural University

Anhui Wanjiang Vegetable Industry Technology Research Institute Co., Ltd.

Lingyun Yuan

Xiaoyan Tang

Chenggang Wang

Zegen Zhang

Jinlong Zhang

Shidong Zhu

Liting Zhang

Xingxue Huang

Jinfeng Hou

The present invention provides a method for improving a one-time seedling rate of microspore embryoids of, including the steps of: spraying 6-BA onto a flower bud of a plant; sterilizing the flower bud with alcohol and HgCl; releasing microspores, filtering and centrifuging to obtain purified microspores; diluting the microspores with a NLN medium, subpackaging into culture dishes, and adding phytic acid; finally, subjecting to heat shock treatment and transferring to culture in the dark until embryoids appear, and then conducting shaking culture; transferring the cultured embryos in a cotyledon stage onto a MS medium for differentiation culture, wherein the culture conditions are: 4&#xb0; C., 14 h of illumination by blue-red compound light/day, and 14 days of culture; and then continuing to culture under the condition of 25&#xb0; C. and 14 h of illumination by blue-red compound light/day until seedlings.

Method for improving one-time seedling rate of microspore embryoids of brassica campestris SSP. chinensis makino

Growing community of inventors

Hefei, China

Lingyun Yuan