Mgi Tech Co., Ltd.

Bgi-shenzhen Co., Limited

Lingyu He

Hui Fu Jiang

Wenwei Zhang

Chunyu Geng

Radoje Drmanac

Yuan Jiang

Xia Zhao

Rongrong Guo

Shujin Fu

Yaqiao Li

Ruoying Chen

Dennis G Ballinger

Andrei Alexeev

Yanyan Zhang

Hui Jiang

Xiaoshan Su

Fanzi Wu

Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3&#x2032; end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3&#x2032; end thereof can be in a complementary pairing with the 5&#x2032; end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.

Linker element and method of using same to construct sequencing library

Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Method for constructing nucleic acid single-stranded cyclic library and reagents thereof

Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagent kit thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5' end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.

Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5'-end nucleotide of the first strand has a phosphate group, and the 3&#x2032;-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5&#x2032;-end nucleotide of the second strand does not have a phosphate group, and the 3&#x2032;-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.

Isolated oligonucleotide and use thereof in nucleic acid sequencing

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Shenzhen, China

Lingyu He