Other

Columbia University

The City University of New York

Robert Alfano

Lingyan Shi

Robert R Alfano

Yang Pu

Sandra Mamani

Yury Budansky

Wei Min

Laura A Sordillo

Binlin Wu

Mikhail Sharonov

Shah Faisal B Mazhar

Sebastião Pratavieira

A Compact Optical Virus Detection Analyzer (COVDA) uses light scattering and fluorescence to detect nanometer (nm) and micrometer (um) sized particles, such as biological particles and can be used to detect viruses such as coronavirus including SAR-CoV-2 responsible for COVID-19, pollen and bacteria. It can be used for prescreening, rapid detection of suspicious people. COVDA involves experimental and theoretical methods for particle and virus detection using Tryptophan as a key biomarker. Light sources in compact units include lamps such as Xenon (Xe) lamp with narrow band filters, LEDs (such as AlN) or laser diode, Q switched and mode lock Lasers for nanosecond and picosecond pulses (such as Nd Yag/Glass, Ti sapphire with Harmonic generator) in blue from 400 nm to 500 nm to generate second harmonic generation (SHG) in KDP/BBO crystals to produce 200 nm to 250 nm emission, or green laser pointers at about 530 nm to get emitters with harmonic crystals at about 270 nm or LEDS from 230 nm to 300 nm for pumping the samples at 230 nm to 289 nm to pump tryptophan and light scatter of nanometer particles of virus. The ultra high power ns and ps lasers in mJ to J can level can be used to locate Bio virus bacteria clouds in free space to image and destroy and kill virus.

Compact optical virus detection analyzer of nano- and micro-size bio particles using light scattering and fluorescence

Incorporation of deuterium into the lipids, proteins, and/or genetic material in cells, tissues, organs, or organisms, including animals, such as humans, and plants, by administration of heavy water, allows for the spectral determination of levels of lipids, proteins, and/or genetic material using Raman spectroscopy, such as stimulated Raman spectroscopy. Following administration of a drug, changes in the relative amounts of lipid, protein, and/or genetic materials can be determined, and the efficacy, or lack thereof, of the administered drug can be determined.

System, method and computer-accessible medium for use of heavy water as a probe for imaging metabolic activities

In this patent, we teach methods to generate coherent X-ray and UUV rays beams for X ray and UUV microscopes using intense femtosecond pulses resulting the Ultra-Supercontinuum (USC) and Higher Harmonic Generation (HHG) from &#x3c7;3 and &#x3c7;media produce from electronic and molecular Kerr effect. The response of n(&#x3c7;3) and n(&#x3c7;5) at the optical frequency from instantaneously response of carrier phase of envelope results in odd HHG and spectral broadening about each harmonic on the anti-Stokes side of the pump pulse at wo typically in the visible, NIR, and MIR. From the slower molecular Kerr response on femtosecond to picosecond from orientation and molecular motion on nand nwhich follow the envelope of optical field of the laser gives rise to extreme broadening without HHG. The resulting spectra extend on the Stokes side towards the IR, RF to DC covering most of the electromagnetic spectrum. These HHG and Super broadening covering UUV to X rays and possibly to gamma ray regime for microscopes.

Table-top ultra supercontinuum and higher harmonic generation source for microscopy

A super class of polarized transverse vector vortex photon beams patterns are mathematically represented here, which are-like among them are the radial and azimuthal Laguerre-Gaussian, hybrid &#x3c0;-vector beams, and Airy beams. These optical beams are consider spin-orbit coupled beams based on OAM and SAM parts of light. Aphoton is a photon that is identical to its anti-photon. It has within itself both chirality, right and left-handed twist in polarization (SAM) and wavefront (OAM). Applications usingphotons improve optical deeper imaging, higher resolution imaging, Nonlinear Optics effects (SHG, SRS, SC), optical communication in free space and fibers, quantum computer as basic qubit, and entanglement for security.

Method for imaging biological tissue using polarized majorana vector and complex vortex photons from laser and supercontinuum light sources

Supercontinuum (SC) (&#x2dc;400 nm to &#x2dc;2500 nm) and a microscope produce enhanced microscopic images on sub-micron to cm scale of linear (&#x3c7;) and nonlinear (&#x3c7;, &#x3c7;, &#x3c7;. . . ) processes via resonance including linear absorption, SHG, THG, SRG, SRL, SRS, 2PEF, 3PEF, 4PEF, and inverse Raman in a microscope for 2D and 3D imaging. Images and processes in 2D and 3D arise from electronic and vibrational resonances transitions in biological and medical tissues, cells, condensed matter applications. Resonant Stimulated Raman Scattering (RSRS) is proposed to improve vibrational imaging of biomaterials by using part of SC. Quantum mechanical processes from SC for 2 and 4 photons to improve resolution and imaging using entangled photons are described. The addition of time measuring instrument like a Streak camera and the scattering coefficient &#x3bc;' can be mapped to create images of tissue and biomaterial in 5D: Space (3D), Time, and Wavelength.

Supercontinuum microscope for resonance and non-resonance enhanced linear and nonlinear images and time resolved microscope for tissues and materials

Majorana photons are transmitted through a biological tissue sample to image the tissue. The Majorana photons have a circular polarization, a radial polarization or an azimuthal polarization. The transmitted photons are processed to produce a digital image of the biological tissue sample.

Method for imaging biological tissue using polarized Majorana photons

There is a need for a compact instrument and microscope that maps the vibration fingerprints of biomolecules and chemicals in a sample such as brain, breast, cervix, and arteries. One can use spontaneous Raman scattering to accomplish this; however, the problem is low scattering efficiency to 10. With the availability of continuous wave diode laser at numerous wavelengths from 375 nm-1800 nm for parametric nonlinear difference vibrational mixing to enhance Stimulated Raman process within materials. A seed beam at Raman frequency is used with pump laser beam. In this way one can map in 2D and 3D images of the vibrations associate with disease changes. Scanning a pair of laser beams can map the location of vibrations within cells, smears, membranes, arteries, and tissues of animal and human.

Continuous diode laser stimulated Raman gain/loss vibrational microscope

A method is provided for deep tissue imaging using multi-photon excitation of a fluorescent agent. The fluorescent agent is irradiated with an ultrafast laser to produce an excited singlet state (S) which subsequently undergoes non-radiative relaxation to a first singlet state (S). The Sstate undergoes fluorescence to the ground state Sto produce an emission wavelength. Both the excitation and emission wavelengths are within the near infrared optical window, thereby permitting deep tissue penetration for both the incoming and outgoing signals.

Method of deep tissue imaging using multi-photon excitation of a fluorophore

A nonlinear optical process on increasing the signal in SRS microscope by Resonant Stimulated Raman Scattering (RSRS) combines both RRS and SRS nonlinear processes, in absorber and host such a &#x3b2;-carotene-methanol solution, Flavins, or other key absorbers in tissues. The observed effect of enhanced RSRS in small signal gain is attributed to RR process in absorber &#x3b2;-carotene that transferring excess vibrations to host methanol from anharmonic vibrational interactions between the solute &#x3b2;-carotene in resonance with the solvent methanol vibrations. SRS microscopy signals are improved in RSRS microscope for imaging vibrational states in lipids CH2 and proteins CH3 in cancer tissues from RR in Flavins or other native chromophores in tissue and for applications in other areas of neuroscience and biomedicine, potentially enhancing signals in the RSRS microscope by 2 to 1000 times.

Resonant stimulated Raman scattering microscope

Coherent light is used to image cells/molecules at wavelengths in the near-infrared (NIR) region in second, third and fourth spectral windows. Optical attenuation from thin tissue slices of normal and malignant breast and prostate tissue, and pig brain are placed between matched bandpass filters, within desired windows and measured within an NIR spectral window at wavelengths selected to highlight the desired cells/molecules. Due to a reduction in scattering and minimal absorption, longer attenuation and clearer images can be seen in the second, third and fourth NIR windows compared to the conventional first NIR window. The spectral windows have uses in microscope imaging one or more collagens, elastins, lipids and carotenoids in arteries, bones, breast, cells, skin, intestines, bones, cracks, teeth, and blood due to less scattering of light and improved signal to noise to provide clearer images.

Deep optical imaging of tissue with less scattering in the second, third and fourth NIR spectral windows using supercontinuum and other laser coherent light sources

Growing community of inventors

San Diego, CA, United States of America

Lingyan Shi