Tsinghua University

Capitalbio Corporation

Targetingone Corporation

Lingxiang Zhu

Can Wang

Jing Cheng

Zhiwei Zhang

Huafang Gao

Qiong Zhang

Bo Wang

Zhe Liu

Di Jiang

Yuxiang Zhou

Wenjun Yang

Yong Guo

Huawei Yang

Ning Du

Shisheng Su

Gaoshan Jing

Miaoqi Zhang

Yongdou Wang

A micro-droplet fluorescence detection system, comprising a microfluidic chip (), an optical path device, and a movement control device for controlling the chip () and an optical path device to move, so that the chip () moves relative to the optical path device during a fluorescence scanning detection process of micro-droplets in the chip (), and the micro-droplets inside the chip () remains stationary relative to the chip (). The micro-droplet fluorescence detection system has advantages of avoiding cross contamination due to closed detection, high detection rate, high throughput and high sensitivity.

Micro-droplet fluorescence detection system

The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5'-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5&#x2032;-terminal of one PCR primer of each pair in extension. With the asymmetric PCR amplification of the invention, high throughput of single-stranded products can be obtained, single PCR amplification or multiple PCR amplification can be carried out. And the method can be widely used in detection of nucleic acids.

Asymmetric PCR amplification, its special primer and application

The present invention provides kits and microarrays containing primer pairs for amplifying drug resistance genes and/or probes for detection of drug resistance genes. Also provided are methods of detecting drug resistance genes using kits and microarrays described herein.

Methods, microarray, and kits for detection of drug resistance genes in gram-negative bacteria

Growing community of inventors

Beijing, China

Lingxiang Zhu